A Doubly Signal-Amplified DNA Detection Method Based on Pre-Complexed [Ru(bpy)3]2+-Doped Silica Nanoparticles

被引：18
作者：
Bae, Se Won [2 ]
Cho, Min Sun [2 ]
Hur, Seong-Suk [3 ]
Chae, Chi-Bom [3 ]
Chung, Doo Soo [2 ]
Yeo, Woon-Seok [1 ]
Hong, Jong-In [2 ]
机构：
[1] Konkuk Univ, Dept Biosci & Biotechnol, Seoul 143701, South Korea
[2] Seoul Natl Univ, Dept Chem, Seoul 151747, South Korea
[3] Konkuk Univ, Inst Biomed Sci & Technol, Seoul 143701, South Korea
来源：
CHEMISTRY-A EUROPEAN JOURNAL | 2010年 / 16卷 / 38期
关键词：
biochips; DNA detection; microarrays; nanoparticles; signal amplification; CATIONIC CONJUGATED POLYMERS; BIOCONJUGATED NANOPARTICLES; MICROARRAYS; PROBES; SENSITIVITY; TECHNOLOGY; SENSORS; CHIP;
D O I：
10.1002/chem.201001255
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Easy detection: The target DNA in a 10-100 am range can be detected by pre-complexed nanoparticles without additional amplification or target labeling. The [Ru(bpy)3]2+-doped silica nanoparticles are hybridized to form a complex with highly enhanced sensitivity (see scheme). This method will be a significant improvement over conventional microarray/ fluorescence readout systems. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：11572 / 11575
页数：4
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