Chemical analyses and anthelmintic effects of Artemisia campestris essential oil

被引:27
作者
Abidi, Amel [1 ,2 ]
Sebai, Essia [1 ,2 ]
Dhibi, Mokhtar [2 ]
Alimi, Dhouha [2 ]
Rekik, Mourad [3 ]
B'chir, Fatma [4 ]
Maizels, R. M. [5 ]
Akkari, Hafidh [2 ]
机构
[1] Univ Tunis El Manar, Fac Sci Tunis, Tunis 2092, Tunisia
[2] Univ Manouba, Lab Parasitol, Ecole Natl Med Vet Sidi Thabet, Manouba 2020, Tunisia
[3] ICARDA, POB 950764, Amman 11195, Jordan
[4] Inst Natl Rech & Anal Phys Chim, Pole Technol Sidi Thabet, Sidi Thabet, Tunisia
[5] Univ Glasgow, Inst Infect Immunol & Inflammat, Trust Ctr Mol Parasitol, Sir Graeme Davies Bldg,120 Univ Pl, Glasgow G12 8TA, Lanark, Scotland
关键词
Artemisia campestris; Haemonchus contortus; Heligmosomoides polygyrus; Anthelmintic activity; HAEMONCHUS-CONTORTUS; IN-VITRO; HELIGMOSOMOIDES-POLYGYRUS; RUTA-CHALEPENSIS; HERBA-ALBA; SHEEP; PLANTS; MICE; RUMINANTS; NEMATODES;
D O I
10.1016/j.vetpar.2018.10.003
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
The present study aimed at analyzing the chemical composition and evaluating the in vitro and in vivo anthelmintic activity of Artemisia campestris essential oil aerial parts. The chemical composition was analysed by gaz chromatography/mass chromatography (GC/MS). Fifty compounds were identified representing 99.98% of the total oil. A. campestris essential oil was dominated by beta-pinene (36.40%) and 2-undecanone (14.7%). The in vitro anthelmintic activity tests of A. campestris essential oil were performed on Haemonchus contortus using egg hatch assay (EHA) and adult worm's motility assay (AWMA) compared with a reference drug al- bendazole. In the EHA 100% inhibition was observed at 2 mg/ml after 48 h incubation (IC50 = 0.93 mg/ml). In the AWMA, essential oil induced 66.6% inhibition at 0.5 mg/ml after 8 h post exposure. The nematicidal effect of essential oil was evaluated on Heligmosomoides polygyrus. It was monitored through faecal egg count reduction (FECR) and total worm count reduction (TWCR). Three doses (2000, 4000 and 5000 mg/kg) were studied using a bioassay. The dose of 5000 mg/kg showed a high nematicidal activity (72.1% FECR and 72% TWCR), 7 days post-treatment. The results of the present study suggest that A. campestris essential oil has a potential anthelmintic activity and further studies are required in order to establish its mechanisms of action.
引用
收藏
页码:59 / 65
页数:7
相关论文
共 51 条
[11]   WORLD-ASSOCIATION-FOR-THE-ADVANCEMENT-OF-VETERINARY-PARASITOLOGY (WAAVP) METHODS FOR THE DETECTION OF ANTHELMINTIC RESISTANCE IN NEMATODES OF VETERINARY IMPORTANCE [J].
COLES, GC ;
BAUER, C ;
BORGSTEEDE, FHM ;
GEERTS, S ;
KLEI, TR ;
TAYLOR, MA ;
WALLER, PJ .
VETERINARY PARASITOLOGY, 1992, 44 (1-2) :35-44
[12]   A NOVEL ANTHELMINTIC MODEL UTILIZING JIRDS, MERIONES-UNGUICULATUS, INFECTED WITH HAEMONCHUS-CONTORTUS [J].
CONDER, GA ;
JEN, LW ;
MARBURY, KS ;
JOHNSON, SS ;
GUIMOND, PM ;
THOMAS, EM ;
LEE, BL .
JOURNAL OF PARASITOLOGY, 1990, 76 (02) :168-170
[13]   GC-MS, GC-O and enantio-GC investigation of the essential oil of Tarchonanthus camphoratus L. [J].
Costa, Rosaria ;
Zellner, Barbara d'Acampora ;
Crupi, Maria L. ;
De Fina, Maria R. ;
Valentino, Maria R. ;
Dugo, Paola ;
Dugo, Giovanni ;
Mondello, Luigi .
FLAVOUR AND FRAGRANCE JOURNAL, 2008, 23 (01) :40-48
[14]  
Dib I., 2017, BMC COMPLEM ALTERN M, V17, P1
[15]   Antimicrobial agents from plants: antibacterial activity of plant volatile oils [J].
Dorman, HJD ;
Deans, SG .
JOURNAL OF APPLIED MICROBIOLOGY, 2000, 88 (02) :308-316
[16]   Thymus capitatus from Tunisian arid zone: Chemical composition and in vitro anthelmintic effects on Haemonchus contortus [J].
Elandalousi, Ramzi Boubaker ;
Akkari, Hafidh ;
B'chir, Fatma ;
Gharbi, Mohamed ;
Mhadhbi, Moez ;
Awadi, Soufia ;
Darghouth, Mohamed Aziz .
VETERINARY PARASITOLOGY, 2013, 197 (1-2) :374-378
[17]   Haemonchus contortus: the then and now, and where to from here? [J].
Emery, David L. ;
Hunt, Peter W. ;
Le Jambre, Leo F. .
INTERNATIONAL JOURNAL FOR PARASITOLOGY, 2016, 46 (12) :755-769
[18]  
Enriquez J.B., 1993, Rec Med Vet., V189, P499
[19]   Antimicrobial and antioxidant properties of Artemisia L. species from western Anatolia [J].
Erel, Sura Baykan ;
Reznicek, Gottfried ;
Senol, Serdar Gokhan ;
Yavasogulu, Nefise Ulku Karabay ;
Konyalioglu, Sibel ;
Zeybek, Ahmet Ulvi .
TURKISH JOURNAL OF BIOLOGY, 2012, 36 (01) :75-84
[20]  
Ghorab H., 2013, Der Pharmacia Lettre, V5, P189