Identification of NCAM that interacts with the PHE-CoV spike protein

被引:21
作者
Gao, Wei [1 ,3 ]
He, Wenqi [1 ]
Zhao, Kui [1 ]
Lu, Huijun [2 ]
Ren, Wenzhi [3 ]
Du, Chongtao [1 ]
Chen, Keyan [1 ]
Lan, Yungang [1 ]
Song, Deguang [1 ]
Gao, Feng [1 ]
机构
[1] Jilin Univ, Coll Anim Sci & Vet Med, Changchun 130062, Peoples R China
[2] Jilin Univ, Inst Zoonosis, Key Lab Zoonosis, Minist Educ, Changchun 130062, Peoples R China
[3] Jilin Univ, Lab Anim Ctr, Changchun 130062, Peoples R China
基金
中国国家自然科学基金;
关键词
HEMAGGLUTINATING ENCEPHALOMYELITIS VIRUS; CELL-ADHESION MOLECULES; ACID-CONTAINING RECEPTORS; INFLUENZA-C VIRUS; AMINOPEPTIDASE-N; BOVINE CORONAVIRUS; N-ACETYL-9-O-ACETYLNEURAMINIC ACID; SIALIC-ACID; PSA-NCAM; INFECTION;
D O I
10.1186/1743-422X-7-254
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Background: The spike proteins of coronaviruses associate with cellular molecules to mediate infection of their target cells. The characterization of cellular proteins required for virus infection is essential for understanding viral life cycles and may provide cellular targets for antiviral therapies. Results: We identified Neural Cell Adhesion Molecule (NCAM) as a novel interacting partner of the PHE-CoV S protein. A T7 phage display cDNA library from N2a cells was constructed, and the library was screened with the soluble PHE-CoV S glycoproteins. We used a coimmunoprecipitation assay to show that only the NCAM was a binding partner of spike protein. We found that a soluble form of anti-NCAM antibody blocked association of the PHE-CoV with N2a cells. Furthermore, double-stranded siRNA targeted against NCAM inhibited PHE-CoV infection. Conclusions: A novel interaction was identified between NCAM and spike protein and this association is critical during PHE-CoV infection.
引用
收藏
页数:11
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