Size separations of starch of different botanical origin studied by asymmetrical-flow field-flow fractionation and multiangle light scattering

被引:34
作者
Wahlund, Karl-Gustav [1 ]
Leeman, Mats [1 ]
Santacruz, Stalin [1 ]
机构
[1] Lund Univ, Fac Engn LTH, Dept Chem, Ctr Chem & Chem Engn, S-22100 Lund, Sweden
关键词
Molar mass; Starch; Size separation; Hydrodynamic diameter; Field-flow fractionation; Multiangle light scattering; INTERMEDIATE MATERIAL; CORN STARCHES; POLYSACCHARIDES; BEHAVIOR; CHANNEL; AMYLOPECTINS; DIMENSIONS; RETENTION; PROTEINS; FEATURES;
D O I
10.1007/s00216-010-4438-5
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Asymmetrical-flow field-flow fractionation combined with multiangle light scattering and refractive index detection has been revealed to be a powerful tool for starch characterization. It is based on size separation according to the hydrodynamic diameter of the starch components. Starch from a wide range of different botanical sources were studied, including normal starch and high-amylose and high-amylopectin starch. The starch was dissolved by heat treatment at elevated pressure in a laboratory autoclave. This gave clear solutions with no granular residues. Amylose retrogradation was prevented by using freshly dissolved samples. Programmed cross flow starting at 1.0 mL min(-1) and decreasing exponentially with a half-life of 4 min was utilised. The starches showed two size populations representing mainly amylose and mainly amylopectin with an overlapping region where amylose and amylopectin were possibly co-eluted. Most of the first population had molar masses below 10(6) g mol(-1), and most of the second size population had molar masses above 10(7) g mol(-1). Large differences were found in the relative amounts of the two populations, the molar mass, and hydrodynamic diameters, depending on the plant source and its varieties.
引用
收藏
页码:1455 / 1465
页数:11
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