Selective intracellular delivery and intracellular recordings combined in MEA biosensors

被引:38
作者
Cerea, Andrea [1 ]
Caprettini, Valeria [1 ]
Bruno, Giulia [1 ,2 ]
Lovato, Laura [1 ]
Melle, Giovanni [1 ,2 ]
Tantussi, Francesco [1 ]
Capozza, Rosario [1 ]
Moia, Fabio [1 ]
Dipalo, Michele [1 ]
De Angelis, Francesco [1 ]
机构
[1] Ist Italiano Tecnol, I-16163 Genoa, Italy
[2] Univ Genoa, DIBRIS, I-16145 Genoa, Italy
基金
欧洲研究理事会;
关键词
CELL-DERIVED CARDIOMYOCYTES; ACTION-POTENTIALS; ELECTROPORATION; NANOSTRAWS; SYSTEM; ARRAY;
D O I
10.1039/c8lc00435h
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Biological studies on in vitro cell cultures are of fundamental importance to investigate cell response to external stimuli, such as new drugs for the treatment of specific pathologies, or to study communication between electrogenic cells. Although three-dimensional (3D) nanostructures brought tremendous improvements on biosensors used for various biological in vitro studies, including drug delivery and electrical recording, there is still a lack of multifunctional capabilities that could help gain deeper insights in several bio-related research fields. In this work, the electrical recording of large cell ensembles and the intracellular delivery of few selected cells are combined on the same device by integrating microfluidic channels on the bottom of a multi-electrode array decorated with 3D hollow nanostructures. The novel platform allows the recording of intracellular-like action potentials from large ensembles of cardiomyocytes derived from human induced pluripotent stem cells (hiPSC) and from the HL-1 line, while different molecules are selectively delivered into single/few targeted cells. The proposed approach shows high potential for enabling new comprehensive studies that can relate drug effects to network level cell communication processes.
引用
收藏
页码:3492 / 3500
页数:9
相关论文
共 28 条
[1]   High-resolution bioelectrical imaging of Aβ-induced network dysfunction on CMOS-MEAs for neurotoxicity and rescue studies [J].
Amin, Hayder ;
Nieus, Thierry ;
Lonardoni, Davide ;
Maccione, Alessandro ;
Berdondini, Luca .
SCIENTIFIC REPORTS, 2017, 7
[2]   Active pixel sensor array for high spatio-temporal resolution electrophysiological recordings from single cell to large scale neuronal networks [J].
Berdondini, Luca ;
Imfeld, Kilian ;
Maccione, Alessandro ;
Tedesco, Mariateresa ;
Neukom, Simon ;
Koudelka-Hep, Milena ;
Martinoia, Sergio .
LAB ON A CHIP, 2009, 9 (18) :2644-2651
[3]  
Boukany PE, 2011, NAT NANOTECHNOL, V6, P747, DOI [10.1038/nnano.2011.164, 10.1038/NNANO.2011.164]
[4]   Nondestructive nanostraw intracellular sampling for longitudinal cell monitoring [J].
Cao, Yuhong ;
Hjort, Martin ;
Chen, Haodong ;
Birey, Fikri ;
Leal-Ortiz, Sergio A. ;
Han, Crystal M. ;
Santiago, Juan G. ;
Pasca, Sergiu P. ;
Wu, Joseph C. ;
Melosh, Nicholas A. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2017, 114 (10) :E1866-E1874
[5]   Soft electroporation for delivering molecules into tightly adherent mammalian cells through 3D hollow nanoelectrodes [J].
Caprettini, Valeria ;
Cerea, Andrea ;
Melle, Giovanni ;
Lovato, Laura ;
Capozza, Rosario ;
Huang, Jian-An ;
Tantussi, Francesco ;
Dipalo, Michele ;
De Angelis, Francesco .
SCIENTIFIC REPORTS, 2017, 7
[6]   3D Hollow Nanostructures as Building Blocks for Multifunctional Plasmonics [J].
De Angelis, Francesco ;
Malerba, Mario ;
Patrini, Maddalena ;
Miele, Ermanno ;
Das, Gobind ;
Toma, Andrea ;
Zaccaria, Remo Proietti ;
Di Fabrizio, Enzo .
NANO LETTERS, 2013, 13 (08) :3553-3558
[7]   Portable cell-based biosensor system using integrated CMOS cell-cartridges [J].
DeBusschere, BD ;
Kovacs, GTA .
BIOSENSORS & BIOELECTRONICS, 2001, 16 (7-8) :543-556
[8]   Characterisation of Connexin Expression and Electrophysiological Properties in Stable Clones of the HL-1 Myocyte Cell Line [J].
Dias, Priyanthi ;
Desplantez, Thomas ;
El-Harasis, Majd A. ;
Chowdhury, Rasheda A. ;
Ullrich, Nina D. ;
de Diego, Alberto Cabestrero ;
Peters, Nicholas S. ;
Severs, Nicholas J. ;
MacLeod, Kenneth T. ;
Dupont, Emmanuel .
PLOS ONE, 2014, 9 (02)
[9]  
Dipalo M, 2018, NAT NANOTECHNOL, V13, P965, DOI 10.1038/s41565-018-0222-z
[10]   Cells Adhering to 3D Vertical Nanostructures: Cell Membrane Reshaping without Stable Internalization [J].
Dipalo, Michele ;
McGuire, Allister F. ;
Lou, Hsin-Ya ;
Caprettini, Valeria ;
Melle, Giovanni ;
Bruno, Giulia ;
Lubrano, Claudia ;
Matino, Laura ;
Li, Xiao ;
De Angelis, Francesco ;
Cui, Bianxiao ;
Santoro, Francesca .
NANO LETTERS, 2018, 18 (09) :6100-6105