Octanoic Acid-Enrichment Diet Improves Endurance Capacity and Reprograms Mitochondrial Biogenesis in Skeletal Muscle of Mice

被引:13
作者
Charlot, Anouk [1 ]
Morel, Lucas [2 ,3 ]
Bringolf, Anthony [1 ]
Georg, Isabelle [1 ]
Charles, Anne-Laure [1 ]
Goupilleau, Fabienne [1 ]
Geny, Bernard [1 ,4 ]
Zoll, Joffrey [1 ,4 ]
机构
[1] Univ Strasbourg, UR 3072 Mitochondrie Stress Oxydant & Protect Mus, Ctr Rech Biomed Strasbourg, F-67000 Strasbourg, France
[2] Luxembourg Inst Hlth, Dept Infect & Immun, L-4354 Esch Sur Alzette, Luxembourg
[3] Univ Luxembourg, Fac Sci Technol & Med, 6 Rue Kalergi, L-1359 Luxembourg, Luxembourg
[4] Hop Univ Strasbourg, Serv Physiol & Explorat Fonct Resp, F-67000 Strasbourg, France
关键词
medium chain fatty acid; octanoic acid; endurance; mitochondrial biogenesis; skeletal muscle; MEDIUM-CHAIN TRIGLYCERIDES; ACTIVATED PROTEIN-KINASE; HIGH-FAT DIET; FIBER-TYPE; EXERCISE INTENSITY; METABOLISM; AMPK; OXIDATION; MECHANISMS; MEN;
D O I
10.3390/nu14132721
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
Background: Medium Chain Fatty Acids (MCFAs) are a dietary supplement that exhibit interesting properties, due to their smaller molecular size. The acute consumption of MCFAs is expected to enhance exercise performance. However, the short-term effects of MCFAs on endurance performance remains poorly understood. The aim of our study is to evaluate the octanoic acid (C8)-rich diet effect on endurance capacity, and to explore their molecular and cellular effects. Methods: C57BL/6J mice were fed with a chow diet (Control group) or an octanoic acid-rich diet (C8 diet) for 6 weeks. Spontaneous activity, submaximal and maximal exercise tests were carried out to characterize the exercise capacities of the mice. Beta-oxidation and mitochondrial biogenesis pathways were explored in skeletal muscle by RT-qPCR, Western Blot (Quadriceps) and histochemical staining (Gastrocnemius). Results: Mice fed with a C8-rich diet presented a higher spontaneous activity (p < 0.05) and endurance capacities (p < 0.05) than the control, but no effect on maximal effort was observed. They also presented changes in the skeletal muscle metabolic phenotype, with a higher number of the oxidative fibers, rich in mitochondria. At the molecular level, the C8-diet induced an AMPK activation (p < 0.05), associated with a significant increase in PGC1a and CS gene expression and protein levels. Conclusion: Our study provided evidence that C8-enrichment as a food supplementation improves endurance capacities and activates mitochondrial biogenesis pathways leading to higher skeletal muscle oxidative capacities.
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页数:14
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共 59 条
[1]  
Bach AC, 1996, J LIPID RES, V37, P708
[2]   The role of medium-chain triglycerides in exercise [J].
Berning, JR .
INTERNATIONAL JOURNAL OF SPORT NUTRITION, 1996, 6 (02) :121-133
[3]  
Bournat Juan C, 2010, Curr Opin Endocrinol Diabetes Obes, V17, P446, DOI 10.1097/MED.0b013e32833c3026
[4]   Ketogenic low-CHO, high-fat diet: the future of elite endurance sport? [J].
Burke, Louise M. .
JOURNAL OF PHYSIOLOGY-LONDON, 2021, 599 (03) :819-843
[5]   Beneficial Effects of the Ketogenic Diet in Metabolic Syndrome: A Systematic Review [J].
Charlot, Anouk ;
Zoll, Joffrey .
DIABETOLOGY, 2022, 3 (02) :292-309
[6]  
College des Enseignants de Nutrition, 2014, NUTR ENS INT UE NUTR
[7]   Fatty acid oxidation is directly regulated by carbohydrate metabolism during exercise [J].
Coyle, EF ;
Jeukendrup, AE ;
Wagenmakers, AJM ;
Saris, WHM .
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 1997, 273 (02) :E268-E275
[8]  
Erickson Anjeza, 2016, Horm Mol Biol Clin Investig, V30, DOI 10.1515/hmbci-2016-0016
[9]  
FUSHIKI T, 1995, J NUTR, V125, P531
[10]   Human skeletal muscle fiber type specific protein content [J].
Galpin, Andrew J. ;
Raue, Ulrika ;
Jemiolo, Bozena ;
Trappe, Todd A. ;
Harber, Matthew P. ;
Minchev, Kiril ;
Trappe, Scott .
ANALYTICAL BIOCHEMISTRY, 2012, 425 (02) :175-182