Providing direction improves function: Comparison of a radial pore-orientated acellular collagen scaffold to clinical alternatives in a surgically induced rabbit diaphragmatic tissue defect model

被引：9

作者：

Eastwood, Mary Patrice ^{[1
,2
]}

Daamen, Willeke F. ^{[3
]}

Joyeux, Luc ^{[1
,2
]}

Pranpanus, Savitree ^{[1
,2
,4
]}

Rynkevic, Rita ^{[1
,5
]}

Hympanova, Lucie ^{[1
,2
,6
]}

Pot, Michiel W. ^{[3
]}

Hof, Danique J. ^{[3
]}

Gayan-Ramirez, Ghislaine ^{[7
]}

van Kuppevelt, Toin H. ^{[3
]}

Verbeken, Eric ^{[8
]}

Deprest, Jan ^{[1
,2
,9
]}

机构：

[1] Katholieke Univ Leuven, Dept Dev & Regenerat, Leuven, Belgium

[2] Katholieke Univ Leuven, Ctr Surg Technol, Leuven, Belgium

[3] Radboud Univ Nijmegen, Med Ctr, Dept Biochem, RIMLS, Nijmegen, Netherlands

[4] Prince Songkla Univ, Dept Obstet & Gynecol, Hat Yai, Thailand

[5] Univ Porto, Fac Engn, NEGI, Porto, Portugal

[6] Charles Univ Prague, Fac Med 3, Inst Care Mother & Child, Prague, Czech Republic

[7] Katholieke Univ Leuven, Lab Pneumol, Leuven, Belgium

[8] Univ Hosp Leuven, Grp Biomed Sci, Dept Pathol, Leuven, Belgium

[9] UCL, Inst Womens Hlth, Res Dept Maternal Fetal Med, London, England

来源：

JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE | 2018年 / 12卷 / 11期

基金：

英国惠康基金; 英国工程与自然科学研究理事会;

关键词：

collagen scaffold; defect repair; diaphragmatic hernia; pore orientation; SMALL-INTESTINAL SUBMUCOSA; PATCH REPAIR; DECELLULARIZED PORCINE; HERNIA REPAIR; CROSS-LINKS; FOLLOW-UP; GORE-TEX; MUSCLE; MATRIX; POLYTETRAFLUOROETHYLENE;

D O I：

10.1002/term.2734

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Gore-Tex (R) is a widely used durable patch for repair of congenital diaphragmatic defects yet may result in complications. We compared Gore-Tex with a composite of a radial pore-orientated collagen scaffold (RP-Composite) and clinically used porcine small intestinal submucosa (SIS; Surgisis (R)) in a rabbit model for diaphragmatic hernia. The growing rabbit mimics the rapid rib cage growth and reherniation rates seen in children. We created and immediately repaired left hemidiaphragmatic defects in 6-week-old rabbits with Gore-Tex, SIS, and an RP-Composite scaffold. An additional group of rabbits had a sham operation. At 90 days, survivors more than doubled in weight. We observed few reherniations or eventrations in Gore-Tex (17%) and RP-Composite (22%) implanted animals. However, SIS failed in all rabbits. Maximum transdiaphragmatic pressure was lower in Gore-Tex (71%) than RP-Composite implanted animals (112%) or sham (134%). Gore-Tex repairs were less compliant than RP-Composite, which behaved as sham diaphragm (p < 0.01). RP-Composite induced less foreign body giant cell reaction than Gore-Tex (p < 0.05) with more collagen deposition (p < 0.001), although there was a tendency for the scaffold to calcify. Unlike Gore-Tex, the compliance of diaphragms reconstructed with RP-Composite scaffolds were comparable with native diaphragm, whereas reherniation rates and transdiaphragmatic pressure measurements were similar.

引用

页码：2138 / 2150

页数：13