An activatable near-infrared fluorescent probe facilitated high-contrast lipophagic imaging in live cells

被引：12

作者：

Chen, Wen ^{[1
]}

Luo, Na ^{[1
]}

Zhang, Yuan ^{[1
]}

Tang, Li-Juan ^{[1
]}

Wang, Fenglin ^{[1
]}

Jiang, Jian-Hui ^{[1
]}

机构：

[1] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2021年 / 57卷 / 69期

基金：

中国国家自然科学基金;

关键词：

LIPID DROPLETS; AUTOPHAGY; ORDER;

D O I：

10.1039/d1cc03259c

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A new fluorescent probe (Q-lipo) was developed by conjugating a xanthene scaffold with a quinoline moiety for activatable imaging of lipophagy. Q-lipo with acidic pH activated near infrared fluorescence and the lipid droplet targeting ability allowed activatable fluorescence imaging and flow cytometry detection of lipophagy in live cells with high contrast. It was further utilized to study the effect of tumor-microenvironment related conditions on lipophagy. Q-lipo would provide a useful tool for studying lipophagy in live cells.

引用

收藏

页码：8664 / 8667

页数：4

相关论文

共 26 条

[1] Single-molecule localization microscopy and tracking with red-shifted states of conventional BODIPY conjugates in living cells [J].

Adhikari, Santosh ;

Moscatelli, Joe ;

Smith, Elizabeth M. ;

Banerjee, Chiranjib ;

Puchner, Elias M. .

NATURE COMMUNICATIONS, 2019, 10 (1)

[2] FoxO1 controls lysosomal acid lipase in adipocytes: implication of lipophagy during nutrient restriction and metformin treatment [J].

Barbato, D. Lettieri ;

Tatulli, G. ;

Aquilano, K. ;

Ciriolo, M. R. .

CELL DEATH & DISEASE, 2013, 4 :e861-e861

[3] Multifunctional Liposome: A Bright AIEgen-Lipid Conjugate with Strong Photosensitization [J].

Cai, Xiaolei ;

Mao, Duo ;

Wang, Can ;

Kong, Deling ;

Cheng, Xiamin ;

Liu, Bin .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2018, 57 (50) :16396-16400

[4] Functions of autophagy in normal and diseased liver [J].

Czaja, Mark J. ;

Ding, Wen-Xing ;

Donohue, Terrence M., Jr. ;

Friedman, Scott L. ;

Kim, Jae-Sung ;

Komatsu, Masaaki ;

Lemasters, John J. ;

Lemoine, Antoinette ;

Lin, Jiandie D. ;

Ou, Jing-Hsiung James ;

Perlmutter, David H. ;

Randall, Glenn ;

Ray, Ratna B. ;

Tsung, Allan ;

Yin, Xiao-Ming .

AUTOPHAGY, 2013, 9 (08) :1131-1158

[5] Targeted Solvatochromic Fluorescent Probes for Imaging Lipid Order in Organelles under Oxidative and Mechanical Stress [J].

Danylchuk, Dmytro, I ;

Jouard, Pierre-Henri ;

Klymchenko, Andrey S. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2021, 143 (02) :912-924

[6] Lipid Droplets Finally Get a Little R-E-S-P-E-C-T [J].

Farese, Robert V., Jr. ;

Walther, Tobias C. .

CELL, 2009, 139 (05) :855-860

[7] Simultaneous Two-Color Visualization of Lipid Droplets and Endoplasmic Reticulum and Their Interplay by Single Fluorescent Probes in Lambda Mode [J].

Guo, Lifang ;

Tian, Minggang ;

Zhang, Zhiyun ;

Lu, Qing ;

Liu, Zhiqiang ;

Niu, Guangle ;

Yu, Xiaoqiang .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2021, 143 (08) :3169-3179

[8] Principles of bioactive lipid signalling: lessons from sphingolipids [J].

Hannun, Yusuf A. ;

Obeid, Lina M. .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2008, 9 (02) :139-150

[9] Long-term live cell microscopy studies of lipid droplet fusion dynamics in adipocytes [J].

Juengst, Christian ;

Klein, Matthias ;

Zumbusch, Andreas .

JOURNAL OF LIPID RESEARCH, 2013, 54 (12) :3419-3429

[10] Switchable Nile Red-Based Probe for Cholesterol and Lipid Order at the Outer Leaflet of Biomembranes [J].

Kucherak, Oleksandr A. ;

Oncul, Sule ;

Darwich, Zeinab ;

Yushchenko, Dmytro A. ;

Arntz, Youri ;

Didier, Pascal ;

Mely, Yves ;

Klymchenko, Andrey S. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2010, 132 (13) :4907-4916