Identification and characterization of two G protein-coupled receptors for neuropeptide FF

被引：381

作者：

Bonini, JA ^{[1
]}

Jones, LK ^{[1
]}

Adham, N ^{[1
]}

Forray, C ^{[1
]}

Artymyshyn, R ^{[1
]}

Durkin, MM ^{[1
]}

Smith, KE ^{[1
]}

Tamm, JA ^{[1
]}

Boteju, LW ^{[1
]}

Lakhlani, PP ^{[1
]}

Raddatz, R ^{[1
]}

Yao, WJ ^{[1
]}

Ogozalek, KL ^{[1
]}

Boyle, N ^{[1
]}

Kouranova, EV ^{[1
]}

Quan, Y ^{[1
]}

Vaysse, PJ ^{[1
]}

Wetzel, JM ^{[1
]}

Branchek, TA ^{[1
]}

Gerald, C ^{[1
]}

Borowsky, B ^{[1
]}

机构：

[1] Synapt Pharmaceut Corp, Paramus, NJ 07652 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2000年 / 275卷 / 50期

关键词：

D O I：

10.1074/jbc.M004385200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The central nervous system octapeptide, neuropeptide FF (NPFF), is believed to play a role in pain modulation and opiate tolerance. Two G protein-coupled receptors, NPFF1 and NPFF2, were isolated from human and rat central nervous system tissues. NPFF specifically bound to NPFF1 (K-d = 1.13 nm) and NPFF2 (K-d = 0.37 nm), and both receptors were activated by NPFF in a variety of heterologous expression systems. The localization of mRNA and binding sites of these receptors in the dorsal horn of the spinal cord, the lateral hypothalamus, the spinal trigeminal nuclei, and the thalamic nuclei supports a role for NPFF in pain modulation. Among the receptors with the highest amino acid sequence homology to NPFF1 and NPFF2 are members of the orexin, NPY, and cholecystokinin families, which have been implicated in feeding. These similarities together with the finding that BIBP3226, an anorexigenic Y1 receptor ligand, also binds to NPFF1 suggest a potential role for NPFF1 in feeding. The identification of NPFF1 and NPFF2 will help delineate their roles in these and other physiological functions.

引用

页码：39324 / 39331

页数：8

共 40 条

[1] AUTORADIOGRAPHIC DISTRIBUTION OF RECEPTORS TO FLFQPQRFAMIDE, A MORPHINE-MODULATING PEPTIDE, IN RAT CENTRAL-NERVOUS-SYSTEM [J].