Single-molecule approach to molecular biology in living bacterial cells

被引：293

作者：

Xie, X. Sunney ^{[1
]}

Choi, Paul J. ^{[1
]}

Li, Gene-Wei ^{[2
]}

Lee, Nam Ki ^{[1
]}

Lia, Giuseppe ^{[1
]}

机构：

[1] Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA

[2] Harvard Univ, Dept Phys, Cambridge, MA 02138 USA

来源：

ANNUAL REVIEW OF BIOPHYSICS | 2008年 / 37卷

关键词：

living cells; stochastic gene expression; DNA-protein interactions; transcription; translation; replication;

D O I：

10.1146/annurev.biophys.37.092607.174640

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Recent developments on fluorescent proteins and microscopy techniques have allowed the probing of single molecules in a living bacteria] cell with high specificity, millisecond time resolution, and nanometer spatial precision. Recording movies and analyzing dynamics of individual macromolecules have brought new insights into the mechanisms of many processes in molecular biology, such is DNA-protein interactions, gene regulation, transcription, translation, and replication, among others. Here we review the key methods of single-molecule detection and highlight numerous examples to illustrate how these experiments are contributing to the quantitative understanding of the fundamental processes in a living cell.

引用

页码：417 / 444

页数：28

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