Establishment of bipotent progenitor cell clone from rat skeletal muscle

被引：9
作者：
Murakami, Yousuke [1 ]
Yada, Erica [1 ]
Nakano, Shin-ichi [1 ]
Miyagoe-Suzuki, Yuko [2 ]
Hosoyama, Tohru [1 ]
Matsuwaki, Takashi [1 ]
Yamanouchi, Keitaro [1 ]
Nishihara, Masugi [1 ]
机构：
[1] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Vet Physiol, Bunkyo Ku, Tokyo 1138657, Japan
[2] Natl Ctr Neurol & Psychiat, Natl Inst Neurosci, Dept Mol Therapy, Kodaira, Tokyo 187, Japan
来源：
ANIMAL SCIENCE JOURNAL | 2011年 / 82卷 / 06期
关键词：
adipogenesis; differentiation; fibroblast growth factor; myogenesis; skeletal muscle; DUCHENNE MUSCULAR-DYSTROPHY; MESENCHYMAL STEM-CELLS; SATELLITE CELLS; ADIPOGENIC DIFFERENTIATION; MYOGENESIS; DISTINCT; ORIGINS; TISSUE;
D O I：
10.1111/j.1740-0929.2011.00907.x
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
The present study describes the isolation, cloning and characterization of adipogenic progenitor cells from rat skeletal muscle. Among the obtained 10 clones, the most highly adipogenic progenitor, 2G11 cells, were further characterized. In addition to their adipogenicity, 2G11 cells retain myogenic potential as revealed by formation of multinucleated myotubes when co-cultured with myoblasts. 2G11 cells were resistant to an inhibitory effect of basic fibroblast growth factor on adipogenesis, while adipogenesis of widely used preadipogenic cell line, 3T3-L1 cells, was suppressed almost completely by the same treatment. In vivo transplantation experiments revealed that 2G11 cells are able to possess both adipogenicity and myogenicity in vivo. These results indicate the presence of bipotent progenitor cells in rat skeletal muscle, and suggest that such cells may contribute to ectopic fat formation in skeletal muscle.
引用
收藏
页码：764 / 772
页数：9
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