Nanoparticle PCR: Nanogold-assisted PCR with enhanced specificity

被引：282

作者：

Li, HK

Huang, JH

Lv, JH

An, HJ

Zhang, XD

Zhang, ZZ ^{[1
]}

Fan, CH

Hu, J

机构：

[1] Shanghai Jiao Tong Univ, BioX DNA Comp Consortium, Shanghai 200030, Peoples R China

[2] Shanghai Jiao Tong Univ, BioX Life Sci Res Ctr, Shanghai 200030, Peoples R China

[3] Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2005年 / 44卷 / 32期

关键词：

DNA replication; gold; nanotechnology; polymerase chain reaction;

D O I：

10.1002/anie.200500403

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Nanogold may not glitter, but its presence significantly improves the specificity and yield of PCR reactions owing to the greater affinity of gold nanoparticles to single-stranded DNA than to double-stranded DNA which helps to reduce mispairing. Indeed, gel electrophoresis shows a single predominant band for the target DNA obtained by this method, in contrast to streaking bands for products obtained by conventional PCR (lanes 1 and 2; see image). (Chemical Equation Presented) © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.

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收藏

页码：5100 / 5103

页数：4

相关论文

共 29 条

[1] FORMATION OF MONOLAYER FILMS BY THE SPONTANEOUS ASSEMBLY OF ORGANIC THIOLS FROM SOLUTION ONTO GOLD [J].

BAIN, CD ;

TROUGHTON, EB ;

TAO, YT ;

EVALL, J ;

WHITESIDES, GM ;

NUZZO, RG .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1989, 111 (01) :321-335

[2]

Bloomfield V. A., 2000, NUCL ACIDS STRUCTURE

[3] Mutation detection by electrocatalysis at DNA-modified electrodes [J].

Boon, EM ;

Ceres, DM ;

Drummond, TG ;

Hill, MG ;

Barton, JK .

NATURE BIOTECHNOLOGY, 2000, 18 (10) :1096-1100

[4] SINGLE-STRANDED-DNA BINDING-PROTEINS REQUIRED FOR DNA-REPLICATION [J].

CHASE, JW ;

WILLIAMS, KR .

ANNUAL REVIEW OF BIOCHEMISTRY, 1986, 55 :103-136

[5] MINIMIZING DELETION MUTAGENESIS ARTIFACT DURING TAQ DNA-POLYMERASE PCR BY ESCHERICHIA-COLI SSB [J].

CHOU, Q .

NUCLEIC ACIDS RESEARCH, 1992, 20 (16) :4371-4371

[6] Thermal desorption behavior and binding properties of DNA bases and nucleosides on gold [J].

Demers, LM ;

Östblom, M ;

Zhang, H ;

Jang, NH ;

Liedberg, B ;

Mirkin, CA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (38) :11248-11249

[7] Direct molecular haplotyping of long-range genomic DNA with M1-PCR [J].

Ding, CM ;

Cantor, CR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (13) :7449-7453

[8] TOUCHDOWN PCR TO CIRCUMVENT SPURIOUS PRIMING DURING GENE AMPLIFICATION [J].

DON, RH ;

COX, PT ;

WAINWRIGHT, BJ ;

BAKER, K ;

MATTICK, JS .

NUCLEIC ACIDS RESEARCH, 1991, 19 (14) :4008-4008

[9] Single-mismatch detection using gold-quenched fluorescent oligonucleotides [J].

Dubertret, B ;

Calame, M ;

Libchaber, AJ .

NATURE BIOTECHNOLOGY, 2001, 19 (04) :365-370

[10] Beyond superquenching: Hyper-efficient energy transfer from conjugated polymers to gold nanoparticles [J].

Fan, CH ;

Wang, S ;

Hong, JW ;

Bazan, GC ;

Plaxco, KW ;

Heeger, AJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (11) :6297-6301