Plasmodium berghei merozoite surface protein-9:: Immunogenicity and protective efficacy using a homologous challenge model

被引：10
作者：
Lopera-Mesa, Tatiana Maria [1 ]
Kushwaha, Ashima [1 ]
Mohmmed, Asif [1 ]
Chauhan, Virander Singh [1 ]
机构：
[1] Int Ctr Genet Engn & Biotechnol, New Delhi 110067, India
来源：
VACCINE | 2008年 / 26卷 / 10期
关键词：
malaria; Plosmodium berghei; merozoite surface protein-9;
D O I：
10.1016/j.vaccine.2007.12.042
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Merozoite surface protein-9 (MSP-9) from Plasmodium is considered a promising vaccine candidate due to its location and possible role in erythrocyte invasion. We report the identification and characterization of Plasmodium berghei MSP-9 (PbMSP-9) and its properties as an immunogen using a recombinant PbMSP-9 fragment to immunize BALB/c mice. PbMSP-9 was found to harbor erythrocyte binding and serine protease activity. PbMSP-9 formulation in alum was highly immunogenic in BALB/c mice. To evaluate the protective efficacy, immunized mice were submitted to homologous challenge with P berghei NK65 btood-stage parasites. Protection against the parasite challenge was observed in BALB/c mice immunized with the PbMSP-9 formulation. These results suggest for the first time that MSP-9 based immunogens may constitute part of an effective malaria vaccine. (C) 2008 Elsevier Ltd. All rights reserved.
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收藏
页码：1335 / 1343
页数：9
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