Genotyping of Toxoplasma gondii isolates from free range chickens in the Pantanal area of Brazil

被引：29
作者：
Soares, Rodrigo Martins [1 ]
Silveira, Luciane Holsback [2 ]
da Silva, Aristeu Vieira [3 ]
Ragozo, Alessandra [1 ]
Galli, Susiana [1 ]
Lopes, Estela Gallucci [1 ]
Gennari, Solange M. [1 ]
de Jesus Pena, Hilda Fatima [1 ]
机构：
[1] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Med Vet Prevent & Saude Anim, BR-05508270 Sao Paulo, Brazil
[2] Univ Estadual Norte Parana, Ctr Ciencias Agr, Setor Patol Geral, BR-86360000 Bandeirantes, PR, Brazil
[3] Univ Estadual Feira de Santana, Dept Ciencias Biol, BR-44036900 Feira De Santana, BA, Brazil
来源：
VETERINARY PARASITOLOGY | 2011年 / 178卷 / 1-2期
关键词：
Toxoplasma gondii; Chicken; Brazil; Genotypes; PCR-RFLP; GENETIC-CHARACTERIZATION; SAO-PAULO; STRAINS; VIRULENCE; DIVERSITY; MARKERS; PARANA; CATS;
D O I：
10.1016/j.vetpar.2010.12.037
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
The aim of this paper was to genetically characterize Toxoplasma gondii isolates from free range chickens in regions of Brazilian territory in the state of Mato Grosso do Sul (MS) where T. gondii strains have never been studied. In total, T. gondii isolates from 22 free range chickens were included in this study. Fifty chickens from Eldorado, thirty from Rio Verde and ten from Aquidauana were sampled between January and April 2007. In relation to the genetic diversity of T. gondii isolates from chickens in MS, the magnitude of the diversity in the isolates sampled in this study was comparable to the overall diversity in a composite data set. These 22 isolates in MS revealed 11 genotypes, whereas the 321 isolates ever genotyped in Brazil have revealed 95 genotypes. The values of Simpson's Diversity Index for the whole population of T. gondii isolates in Brazil, the whole population of T. gondii isolates from chickens in Brazil and the population surveyed in this study were 0.97, 0.95 and 0.90, respectively. Seven of the 11 genotypes revealed from chicken isolates from MS are newly described genotypes and six of them each have a single isolate. In conclusion, the results obtained from isolates in MS corroborate previous studies on T. gondii isolates in Brazil, thus confirming their diversity and atypicality. Nonetheless, the applicability of PCR-RFLP markers for epidemiological inferences remains controversial. (c) 2011 Elsevier B.V. All rights reserved.
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页码：29 / 34
页数：6
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