Cell surface and intracellular auxin signalling for H+ fluxes in root growth

被引：183

作者：

Li, Lanxin ^{[1
]}

Verstraeten, Inge ^{[1
]}

Roosjen, Mark ^{[2
]}

Takahashi, Koji ^{[3
,4
]}

Rodriguez, Lesia ^{[1
]}

Merrin, Jack ^{[1
]}

Chen, Jian ^{[5
,6
]}

Shabala, Lana ^{[7
]}

Smet, Wouter ^{[5
,6
]}

Ren, Hong ^{[8
]}

Vanneste, Steffen ^{[5
,9
,10
]}

Shabala, Sergey ^{[7
,11
]}

De Rybel, Bert ^{[5
,6
]}

Weijers, Dolf ^{[2
]}

Kinoshita, Toshinori ^{[3
,4
]}

Gray, William M. ^{[8
]}

Friml, Jiri ^{[1
]}

机构：

[1] IST Austria, Klosterneuburg, Austria

[2] Wageningen Univ, Dept AgrotechnoL & Food Sci, Lab Biochem, Wageningen, Netherlands

[3] Nagoya Univ Chikusa, Inst Transformat Biomol, Div Biol Sci, Nagoya, Aichi, Japan

[4] Nagoya Univ Chikusa, Grad Sch Sci, Nagoya, Aichi, Japan

[5] Univ Ghent, Dept Plant Biotechnol & Bioinformat, Ghent, Belgium

[6] VIB Ctr Plant Syst Biol, Ghent, Belgium

[7] Univ Tasmania, Tasmanian Inst Agr, Coll Sci & Engn, Hobart, Tas, Australia

[8] Univ Minnesota, Dept Plant & Microbial Biol, St Paul, MN USA

[9] Univ Ghent, Lab Plant Growth Anal, Global Campus, Incheon, South Korea

[10] Univ Ghent, Dept Plants & Crops, HortiCell, Ghent, Belgium

[11] Foshan Univ, Int Res Ctr Environm Membrane Biol, Foshan, Peoples R China

来源：

NATURE | 2021年 / 599卷 / 7884期

基金：

欧盟地平线“2020”; 美国国家卫生研究院; 欧洲研究理事会; 奥地利科学基金会; 澳大利亚研究理事会;

关键词：

GENE; ATPASE; PHOSPHORYLATION; INHIBITION; ENDOCYTOSIS; ELONGATION; EXPANSION; PLANTS; FAMILY; CA2+;

D O I：

10.1038/s41586-021-04037-6

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Growth regulation tailors development in plants to their environment. A prominent example of this is the response to gravity, in which shoots bend up and roots bend down(1). This paradox is based on opposite effects of the phytohormone auxin, which promotes cell expansion in shoots while inhibiting it in roots via a yet unknown cellular mechanism(2). Here, by combining microfluidics, live imaging, genetic engineering and phosphoproteomics in Arabidopsis thaliana, we advance understanding of how auxin inhibits root growth. We show that auxin activates two distinct, antagonistically acting signalling pathways that converge on rapid regulation of apoplastic pH, a causative determinant of growth. Cell surface-based TRANSMEMBRANE KINASE1 (TMK1) interacts with and mediates phosphorylation and activation of plasma membrane H+-ATPases for apoplast acidification, while intracellular canonical auxin signalling promotes net cellular H+ influx, causing apoplast alkalinization. Simultaneous activation of these two counteracting mechanisms poises roots for rapid, fine-tuned growth modulation in navigating complex soil environments.

引用

页码：273 / +

页数：22

共 64 条

[1] Reorientation of Cortical Microtubule Arrays in the Hypocotyl of Arabidopsis thaliana Is Induced by the Cell Growth Process and Independent of Auxin Signaling [J].