Detection of noncovalent interactions of hairpin oligonucleotide with stilbazolium ligands by MALDI TOF mass spectrometry

被引：4

作者：

Juskowiak, B ^{[1
]}

Chudak, M

Takenaka, S

机构：

[1] Adam Mickiewicz Univ, Fac Chem, Dept Analyt Chem, PL-60780 Poznan, Poland

[2] Kyushu Univ, Fac Engn, Dept Appl Chem, Fukuoka 8128581, Japan

来源：

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY | 2003年 / 229卷 / 03期

关键词：

MALDI TOF mass spectrometry; noncovalent interactions; oligonucleotides; stilbazolium ligands;

D O I：

10.1016/S1387-3806(03)00340-3

中图分类号：

O64 [物理化学（理论化学）、化学物理学]; O56 [分子物理学、原子物理学];

学科分类号：

070203 ; 070304 ; 081704 ; 1406 ;

摘要：

Matrix-assisted laser desorption ionization time-of-flight (MALDI TOF) mass spectroscopic analysis was carried out to study the noncovalent complexes of aryl stilbazolium ligands with short deoxyoligonucleotide, 5'CGCTTTGCG-3' (sT(3)). The method enables to assess rapidly stoichiometries of complexes. The number of peaks associated with complexes and their relative abundances depended on the structure of aryl moiety of the ligand and reflected DNA-binding mode and affinity of the ligand. The most potent ligands, 9-[2-(N-methylpyridinium-4-yl) vinyl] anthracene and 2- [2-(N-methylpyridinium-4-yl)vinyl] naphthalene formed 1:1, 1:2 and 1:3 complexes with both hairpin and bulged duplex structures of sT(3) oligonucleotide. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：225 / 230

页数：6

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