A conformationally purified α-helical peptide library

被引：28

作者：

Fujii, I ^{[1
]}

Takaoka, Y ^{[1
]}

Suzuki, K ^{[1
]}

Tanaka, T ^{[1
]}

机构：

[1] Biomol Engn Res Inst, Osaka 5650874, Japan

来源：

TETRAHEDRON LETTERS | 2001年 / 42卷 / 19期

关键词：

helix-loop helix; affinity chromatography; peptide library; immobilized metal-affinity chromatography;

D O I：

10.1016/S0040-4039(01)00382-3

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A conformationally purified peptide library was constructed based on a unique idea combining the combinatorial library concept with de novo peptide design. This peptide library was designed to allow peptides to acquire metal-binding ability when they fold into helix-loop helix structures. Therefore, a mixture of randomized peptides was purified by immobilized metal-affinity chromatography (IMAC) to provide a library of peptides with the homogenous conformation. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：3323 / 3325

页数：3

共 16 条

[1] ENGINEERED METAL-BINDING PROTEINS - PURIFICATION TO PROTEIN FOLDING
ARNOLD, FH
HAYMORE, BL
[J]. SCIENCE, 1991, 252 (5014) : 1796 - 1797
[2] UNRAVELING THE STRUCTURE OF IL-2
BAZAN, JF
[J]. SCIENCE, 1992, 257 (5068) : 410 - 412
[3] HUMAN GROWTH-HORMONE AND EXTRACELLULAR DOMAIN OF ITS RECEPTOR - CRYSTAL-STRUCTURE OF THE COMPLEX
DEVOS, AM
ULTSCH, M
KOSSIAKOFF, AA
[J]. SCIENCE, 1992, 255 (5042) : 306 - 312
[4] EICHLER J, 1994, PEPTIDE RES, V7, P300
[5] SECONDARY STRUCTURE NUCLEATION IN PEPTIDES - TRANSITION-METAL ION STABILIZED ALPHA-HELICES
GHADIRI, MR
CHOI, C
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1990, 112 (04) : 1630 - 1632
[6] Use of a conformationally restricted secondary structural element to display peptide libraries: A two-stranded alpha-helical coiled-coil stabilized by lactam bridges
Houston, ME
Wallace, A
Bianchi, E
Pessi, A
Hodges, RS
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1996, 262 (02) : 270 - 282
[7] KOSSIAKOFF AA, 1992, SCIENCE, V255, P312
[8] A NEW TYPE OF SYNTHETIC PEPTIDE LIBRARY FOR IDENTIFYING LIGAND-BINDING ACTIVITY
LAM, KS
SALMON, SE
HERSH, EM
HRUBY, VJ
KAZMIERSKI, WM
KNAPP, RJ
[J]. NATURE, 1991, 354 (6348) : 82 - 84
[9] TENDAMISTAT AS A SCAFFOLD FOR CONFORMATIONALLY CONSTRAINED PHAGE PEPTIDE LIBRARIES
MCCONNELL, SJ
HOESS, RH
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1995, 250 (04) : 460 - 470
[10] PerezPaya E, 1996, J BIOL CHEM, V271, P4120

← 1 2 →