Integrated metabolomic profiling for analysis of antilipidemic effects of Polygonatum kingianum extract on dyslipidemia in rats

被引：25

作者：

Yang, Xing-Xin ^{[1
]}

Wei, Jia-Di ^{[1
]}

Mu, Jian-Kang ^{[1
]}

Liu, Xin ^{[2
]}

Dong, Jin-Cai ^{[1
]}

Zeng, Lin-Xi ^{[1
]}

Gu, Wen ^{[1
]}

Li, Jing-Ping ^{[1
]}

Yu, Jie ^{[1
]}

机构：

[1] Yunnan Univ Tradit Chinese Med, Coll Pharmaceut Sci, 1076 Yuhua Rd, Kunming 650500, Yunnan, Peoples R China

[2] Beijing Entry Exit Inspect & Quarantine Bur, Beijing 100026, Peoples R China

来源：

WORLD JOURNAL OF GASTROENTEROLOGY | 2018年 / 24卷 / 48期

基金：

中国国家自然科学基金;

关键词：

Dyslipidemia; Lipid regulation; Metabolomics; Multivariate statistical analysis; Polygonatum kingianum; Ultra-high performance liquid chromatography/mass spectrometry; TOTAL SAPONINS; PROGRESS; ACID;

D O I：

10.3748/wjg.v24.i48.5505

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

AIM To identify the effects and mechanism of action of Polygonatum kingianum (P. kingianum) on dyslipidemia in rats using an integrated untargeted metabolomic method. METHODS A rat model of dyslipidemia was induced with a high-fat diet (HFD) and rats were given P, kingianum [4 g/(kg.d)] intragastrically for 14 wk. Changes in serum and hepatic lipid parameters were evaluated. Metabolites in serum, urine and liver samples were profiled using ultra-high performance liquid chromatography/mass spectrometry followed by multivariate statistical analysis to identify potential biomarkers and metabolic pathways. RESULTS P. kingianum significantly inhibited the HFD-induced increase in total cholesterol and triglyceride in the liver and serum. P. kingianum also significantly regulated metabolites in the analyzed samples toward normal status. Nineteen, twenty-four and thirty-eight potential biomarkers were identified in serum, urine and liver samples, respectively. These biomarkers involved biosynthesis of phenylalanine, tyrosine, tryptophan, valine, leucine and isoleucine, along with metabolism of tryptophan, tyrosine, phenylalanine, starch, sucrose, glycerophospholipid, arachidonic acid, linoleic acid, nicotinate, nicotinamide and sphingolipid. CONCLUSION P. kingianum alleviates HFD-induced dyslipidemia by regulating many endogenous metabolites in serum, urine and liver samples. Collectively, our findings suggest that P. kingianum may be a promising lipid regulator to treat dyslipidemia and associated diseases.

引用

页码：5505 / 5524

页数：20

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