Structural characterization of an immunoenhancing heteroglycan isolated from an aqueous extract of an edible mushroom, Lentinus squarrosulus (Mont.) Singer

被引：46

作者：

Bhunia, Sanjoy Kumar ^{[1
]}

Dey, Biswajit ^{[1
]}

Maity, Kankan K. ^{[1
]}

Patra, Sukesh ^{[1
]}

Mandal, Soumitra ^{[1
]}

Maiti, Swatilekha ^{[2
]}

Maiti, Tapas K. ^{[2
]}

Sikdar, Samir R. ^{[3
]}

Islam, Syed S. ^{[1
]}

机构：

[1] Vidyasagar Univ, Dept Chem & Chem Technol, Midnapore 721102, West Bengal, India

[2] Indian Inst Technol IIT Kharagpur, Dept Biotechnol, Kharagpur 721302, West Bengal, India

[3] Bose Inst, Plant Mol & Cellular Genet Sect, Kolkata 700054, West Bengal, India

来源：

CARBOHYDRATE RESEARCH | 2010年 / 345卷 / 17期

关键词：

Lentinus squarrosulus (Mont.) Singer; Polysaccharide; Structure; NMR spectroscopy; Splenocyte and thymocyte activation; WATER-SOLUBLE GLUCAN; ASTRAEUS-HYGROMETRICUS; PLEUROTUS-FLORIDA; FRUIT-BODIES; (1->3)-BETA-D-GLUCAN; ELUCIDATION; ANTITUMOR;

D O I：

10.1016/j.carres.2010.09.015

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A water-soluble polysaccharide, isolated from the hot water extract of the fruiting bodies of an edible mushroom. Lentinus squarrosulus (Mont.) Singer was found to contain D-galactose, L-fucose, and D-glucose in a molar ratio of nearly 1:1:5. Structural investigation of the polysaccharide was carried out using acid hydrolysis, methylation analysis, periodate oxidation study, and NMR experiments (H-1, C-13, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC). On the basis of above-mentioned experiments, the structure of the repeating unit of the polysaccharide was established as: [GRAPHICS] This polysaccharide showed macrophage as well as splenocyte and thymocyte activation. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：2542 / 2549

页数：8

共 33 条

[1] USE OF LITHIUM ALUMINIUM HYDRIDE IN THE STUDY OF CARBOHYDRATES [J].

ABDELAKHER, M ;

SMITH, F .

NATURE, 1950, 166 (4233) :1037-1038

[2] NMR-SPECTROSCOPY IN THE STRUCTURAL ELUCIDATION OF OLIGOSACCHARIDES AND GLYCOSIDES [J].

AGRAWAL, PK .

PHYTOCHEMISTRY, 1992, 31 (10) :3307-3330

[3]

Akpaja Emmanuel O., 2003, International Journal of Medicinal Mushrooms, V5, P313, DOI 10.1615/InterJMedicMush.v5.i3.100

[4] Structural investigation of a water-soluble glucan from an edible mushroom, Astraeus hygrometricus [J].

Chakraborty, I ;

Mondal, S ;

Pramanik, M ;

Rout, D ;

Islam, SS .

CARBOHYDRATE RESEARCH, 2004, 339 (13) :2249-2254

[5] Structural investigation of a heteroglycan isolated from the fruit bodies of an ectomycorrhizal fungus Astraeus hygrometricus [J].

Chakraborty, Indranil ;

Mondal, Soumitra ;

Rout, Dilip ;

Chandra, Krishnendu ;

Islam, Syed S. .

CARBOHYDRATE RESEARCH, 2007, 342 (07) :982-987

[6] A water-soluble glucan isolated from an edible mushroom Termitomyces microcapus [J].

Chandra, Krishnendu ;

Ghosh, Kaushik ;

Roy, Sadhan K. ;

Mondal, Subtlas ;

Maiti, Debabrata ;

Ojha, Arnab K. ;

Das, Debsankar ;

Mondal, Soumitra ;

Islam, Syed S. .

CARBOHYDRATE RESEARCH, 2007, 342 (16) :2484-2489

[7]

CHIHARA G, 1969, Nature (London), V222, P687

[8]

Chihara G., 1978, MUSHROOM SCI, V9, P797

[9] A SIMPLE AND RAPID METHOD FOR THE PERMETHYLATION OF CARBOHYDRATES [J].

CIUCANU, I ;

KEREK, F .

CARBOHYDRATE RESEARCH, 1984, 131 (02) :209-217

[10]

Duenas Chaso M. T., 1997, CARBOHYD RES, V303, P453

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