GENE EXPRESSION ANALYSIS OF MOUSE EMBRYONIC STEM CELLS FOLLOWING LEVITATION IN AN ULTRASOUND STANDING WAVE TRAP

被引:44
作者
Bazou, Despina [1 ]
Kearney, Roisin [2 ]
Mansergh, Fiona [3 ]
Bourdon, Celine [2 ]
Farrar, Jane [3 ]
Wride, Michael [2 ]
机构
[1] Trinity Coll Dublin, CRANN, Dublin 2, Ireland
[2] Trinity Coll Dublin, Dept Zool, Dublin 2, Ireland
[3] Trinity Coll Dublin, Smurfit Inst Genet, Dublin 2, Ireland
基金
英国惠康基金;
关键词
Embryonic stem cells; Embryoid bodies; Gene expression; Differentiation; Neural; Pluripotency; Ultrasound; Cell manipulation; Microenvironment; PLURIPOTENCY; ORGANIZATION; NICHES; DEVICE;
D O I
10.1016/j.ultrasmedbio.2010.10.019
中图分类号
O42 [声学];
学科分类号
070206 ; 082403 ;
摘要
In the present paper, gene expression analysis of mouse embryonic stem (ES) cells levitated in a novel ultrasound standing wave trap (USWT) (Bazou et al. 2005a) at variable acoustic pressures (0.08-0.85 MPa) and times (5-60 min) was performed. Our results showed that levitation of ES cells at the highest employed acoustic pressure for 60 min does not modify gene expression and cells maintain their pluripotency. Embryoid bodies (EBs) also expressed the early and late neural differentiation markers, which were also unaffected by the acoustic field. Our results suggest that the ultrasound trap microenvironment is minimally invasive as the biologic consequences of ES cell replication and EB differentiation proceed without significantly affecting gene expression. The technique holds great promise in safe cell manipulation techniques for a variety of applications including tissue engineering and regenerative medicine. (E-mail: Bazoud@tcd.ie) (C) 2011 World Federation for Ultrasound in Medicine & Biology.
引用
收藏
页码:321 / 330
页数:10
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