GENE EXPRESSION ANALYSIS OF MOUSE EMBRYONIC STEM CELLS FOLLOWING LEVITATION IN AN ULTRASOUND STANDING WAVE TRAP

被引：44
作者：
Bazou, Despina [1 ]
Kearney, Roisin [2 ]
Mansergh, Fiona [3 ]
Bourdon, Celine [2 ]
Farrar, Jane [3 ]
Wride, Michael [2 ]
机构：
[1] Trinity Coll Dublin, CRANN, Dublin 2, Ireland
[2] Trinity Coll Dublin, Dept Zool, Dublin 2, Ireland
[3] Trinity Coll Dublin, Smurfit Inst Genet, Dublin 2, Ireland
来源：
ULTRASOUND IN MEDICINE AND BIOLOGY | 2011年 / 37卷 / 02期
基金：
英国惠康基金;
关键词：
Embryonic stem cells; Embryoid bodies; Gene expression; Differentiation; Neural; Pluripotency; Ultrasound; Cell manipulation; Microenvironment; PLURIPOTENCY; ORGANIZATION; NICHES; DEVICE;
D O I：
10.1016/j.ultrasmedbio.2010.10.019
中图分类号：
O42 [声学];
学科分类号：
070206 ; 082403 ;
摘要：
In the present paper, gene expression analysis of mouse embryonic stem (ES) cells levitated in a novel ultrasound standing wave trap (USWT) (Bazou et al. 2005a) at variable acoustic pressures (0.08-0.85 MPa) and times (5-60 min) was performed. Our results showed that levitation of ES cells at the highest employed acoustic pressure for 60 min does not modify gene expression and cells maintain their pluripotency. Embryoid bodies (EBs) also expressed the early and late neural differentiation markers, which were also unaffected by the acoustic field. Our results suggest that the ultrasound trap microenvironment is minimally invasive as the biologic consequences of ES cell replication and EB differentiation proceed without significantly affecting gene expression. The technique holds great promise in safe cell manipulation techniques for a variety of applications including tissue engineering and regenerative medicine. (E-mail: Bazoud@tcd.ie) (C) 2011 World Federation for Ultrasound in Medicine & Biology.
引用
收藏
页码：321 / 330
页数：10
相关论文
共 31 条
[1]   Effects of Ethanol on Mouse Embryonic Stem Cells [J].
Arzumanyan, Alla ;
Anni, Helen ;
Rubin, Raphael ;
Rubin, Emanuel .
ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH, 2009, 33 (12) :2172-2179
[2]   EMBRYONIC STEM-CELLS EXPRESS NEURONAL PROPERTIES IN-VITRO [J].
BAIN, G ;
KITCHENS, D ;
YAO, M ;
HUETTNER, JE ;
GOTTLIEB, DI .
DEVELOPMENTAL BIOLOGY, 1995, 168 (02) :342-357
[3]   Long-term viability and proliferation of alginate-encapsulated 3-D HepG2 aggregates formed in an ultrasound trap [J].
Bazou, D. ;
Coakley, W. T. ;
Hayes, A. J. ;
Jackson, S. K. .
TOXICOLOGY IN VITRO, 2008, 22 (05) :1321-1331
[4]   Molecular adhesion development in a neural cell monolayer forming in an ultrasound trap [J].
Bazou, D ;
Foster, GA ;
Ralphs, JR ;
Coakley, WT .
MOLECULAR MEMBRANE BIOLOGY, 2005, 22 (03) :229-240
[5]   Physical enviroment of 2-D animal cell aggregates formed in a short pathlength ultrasound standing wave trap [J].
Bazou, D ;
Kuznetsova, LA ;
Coakley, WT .
ULTRASOUND IN MEDICINE AND BIOLOGY, 2005, 31 (03) :423-430
[6]   Gap junctional intercellular communication and cytoskeletal organization in chondrocytes in suspension in an ultrasound trap [J].
Bazou, Despina ;
Dowthwaite, Gary P. ;
Khan, Ilyas M. ;
Archer, Charles W. ;
Ralphs, James R. ;
Coakley, W. Terence .
MOLECULAR MEMBRANE BIOLOGY, 2006, 23 (02) :195-205
[7]   Generation of a defined and uniform population of CNS progenitors and neurons from mouse embryonic stem cells [J].
Bibel, Miriam ;
Richter, Jens ;
Lacroix, Emmanuel ;
Barde, Yves-Alain .
NATURE PROTOCOLS, 2007, 2 (05) :1034-1043
[8]   Engineering the Embryoid Body Microenvironment to Direct Embryonic Stem Cell Differentiation [J].
Bratt-Leal, Andres M. ;
Carpenedo, Richard L. ;
McDevitt, Todd C. .
BIOTECHNOLOGY PROGRESS, 2009, 25 (01) :43-51
[9]  
BUSTAMANTE C, 2009, CSH PROTOC, V10
[10]  
Coakley WT, 2004, COLLOID SURFACE B, V34, P221, DOI [10.1016/j.colsurfb.2004.01.002, 10.1016/j.colfsurb.2004.01.002]
1234