Autoantibodies Against Glycoprotein 2 Isoforms in Pediatric Patients with Inflammatory Bowel Disease

被引:12
作者
Roeber, Nadja [1 ]
Noss, Lydia [2 ]
Goihl, Alexander [3 ]
Reinhold, Dirk [3 ]
Jahn, Josefine [2 ]
de laffolie, Jan [4 ]
Johannes, Wolf [5 ,6 ]
Flemming, Gunter M. [7 ]
Roggenbuck, Dirk [8 ,9 ,10 ,11 ]
Conrad, Karsten [1 ]
Laass, Martin W. [2 ]
机构
[1] Tech Univ Dresden, Med Fac Carl Gustav Carus, Inst Immunol, Dresden, Germany
[2] Tech Univ Dresden, Childrens Hosp, Med Fac Carl Gustav Carus, Dresden, Germany
[3] Otto von Guericke Univ, Inst Mol & Clin Immunol, Magdeburg, Germany
[4] Justus Liebig Univ Giessen, Childrens Hosp, Giessen, Germany
[5] Univ Leipzig, Med Fac, Inst Lab Med Clin Chem & Mol Diagnost, Leipzig, Germany
[6] Univ Hosp, Leipzig, Germany
[7] Univ Childrens Hosp Leipzig, Leipzig, Germany
[8] GA Gener Assays GmbH, Dahlewitz Berlin, Germany
[9] Brandenburg Univ Technol Cottbus Senftenberg, Fac Environm & Nat Sci, Inst Biotechnol, Senftenberg, Germany
[10] Gener Assays GmbH, Dahlewitz Berlin, Germany
[11] Medipan GmbH, Blankenfelde Mahlow, Germany
关键词
inflammatory bowel disease; Crohn's disease; GP2; autoantibodies; pediatric; CROHNS-DISEASE; PANCREATIC AUTOANTIBODIES; CLINICAL-SIGNIFICANCE; ANTI-GP2; ANTIBODIES; MARKERS; DIAGNOSIS; GP2; CLASSIFICATION; IDENTIFICATION; CHILDREN;
D O I
10.1097/MIB.0000000000001159
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Background: Anti-Glycoprotein 2 (GP2) antibodies are associated with a more complicated course of Crohn's disease (CD) in adults. Four different GP2 isoforms with different length and antibody-binding sites have been identified so far but not been explored in serological studies. We aimed to investigate the diagnostic utility of autoantibodies against all 4 isoforms of GP2 in an exclusively pediatric population for the first time. Methods: We included 278 children and adolescents with inflammatory bowel disease: 164 with CD, 114 with ulcerative colitis, 83 disease controls (acute gastrointestinal infection, nonspecific gastrointestinal functional disorders), and 219 healthy controls. Sera were tested for anti-GP2 antibodies using 4 different isoforms of GP2 for anti-Saccharomyces cerevisiae antibodies, antineutrophil cytoplasmic antibodies, and pancreatic antibodies. Results: Anti-GP2 antibodies were significantly more prevalent in patients with CD than in ulcerative colitis and controls. We found a sensitivity of 38% (with a specificity of 95%) for anti-GP2 IgG against isoform 4 in CD. Anti-GP2 IgA against isoform 1 and anti-GP2 IgG against isoform 4 possessed the best diagnostic values for identification of CD. For the differentiation of CD from ulcerative colitis anti-GP2 IgG against isoforms 3 and 4 proved to be most accurate markers. Anti-GP2 antibodies were associated with a more complicated disease behavior and bowel surgery in CD. In a subgroup of patients with CD, anti-GP2 IgG against isoform 4 proved to be a relatively stable marker over time independent of disease activity. Conclusions: Anti-GP2 antibodies against different isoforms are specific markers for CD and for different phenotypes in pediatric inflammatory bowel disease.
引用
收藏
页码:1624 / 1636
页数:13
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