A DNA-Origami Chip Platform for Label-Free SNP Genotyping Using Toehold-Mediated Strand Displacement

被引：116

作者：

Zhang, Zhao ^{[1
,4
]}

Zeng, Dongdong ^{[2
,3
]}

Ma, Hongwei ^{[3
]}

Feng, Guoying ^{[1
]}

Hu, Jun ^{[2
]}

He, Lin ^{[1
]}

Li, Can ^{[1
]}

Fan, Chunhai ^{[1
,2
]}

机构：

[1] Shanghai Jiao Tong Univ, Key Lab Genet Dev & Neuropsychiat Disorders, Minist Educ, BioX Ctr, Shanghai 200030, Peoples R China

[2] Chinese Acad Sci, Phys Biol Lab, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China

[3] Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou 215125, Peoples R China

[4] Aarhus Univ, Ctr DNA Nanotechnol CDNA, DK-8000 Aarhus C, Denmark

来源：

SMALL | 2010年 / 6卷 / 17期

关键词：

CHRONOCOULOMETRIC DNA; NANOPARTICLE; SENSOR; RNA;

D O I：

10.1002/smll.201000908

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Single-nucleotide polymorphisms (SNPs) are the most abundant genetic variations in the human genome [1] and hold great promise as disease-associated biomarkers.[2] There have been a variety of SNP genotyping methods, including primer extension, hybridization, ligation, and enzymatic cleavage with optical or electronic readout.[3-13] Despite this, much effort has been expended recently to develop simple and effective SNP-genotyping methods, preferably free of target labeling, without the need of stringent control of assay conditions and/or tackling complex enzyme reactions. In addition, chip-based SNP genotyping methods often suffer from relatively slow kinetics and low hybridization effi ciency at heterogeneous interfaces. [14] In this paper, we report a "DNA origami"-based strategy for label-free SNP detection in homogeneous solutions ( Figure 1 ).Copyright © 2010 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：1854 / 1858

页数：5

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