Study of innervation, sensory neuropeptides, and serotonin in murine contact allergic skin

被引:16
作者
El-Nour, H [1 ]
Lundeberg, L
Boman, A
Beck, O
Harvima, IT
Theodorsson, E
Nordlind, K
机构
[1] Karolinska Univ Hosp, Unit Dermatol & Venerol, Dept Med, SE-17176 Stockholm, Sweden
[2] Dept Med, Unit Occupat & Environm Dermatol, Stockholm, Sweden
[3] Karolinska Univ Hosp, Clin Pharmacol Unit, Dept Med, SE-17176 Stockholm, Sweden
[4] Kuopio Univ Hosp, Dept Dermatol, SF-70210 Kuopio, Finland
[5] Linkoping Univ Hosp, IBK Neurochem, S-58185 Linkoping, Sweden
关键词
axonal growth; contact allergy; inflammation; mouse; nerve fibers; sensory neuropeptides; serotonin;
D O I
10.1081/IPH-200051617
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Density of nerve fibers, axonal growth, calcitonin gene-related peptide (CGRP), and substance P, and serotonin immunoreactivity as well as concentration were all determined in a murine model of contact allergy. Female Balb/c mice were sensitized on the back with oxazolone and 6 days later challenged with the same antigen on the dorsal surface of the ears, while control mice received the vehicle only. Then, 24 hr postchallenge, one ear was processed for immunohistochemical staining, while the other was frozen and processed for gas chromatography-mass spectrometry or radioimmunoassay (RIA). Protein gene product 9.5 (PGP 9.5) positive nerve fibers showed a tendency to increase in inflamed ears versus control ears in epidermis as well as the dermis. Growth-associated protein-43 (GAP-43) positive fibers in the epidermis were increased (p < .01) in inflamed ears, compared with control ears, as was the case for the dermal fibers, indicating increased axonal growth. Total (epidermis and dermis) numbers of CGRP and substance P positive nerve fibers tended to increase in the inflamed skin in contrast to control skin. In contrast, RIA demonstrated a lower (p < .05) concentration of CGRP in the inflamed ears compared with controls and a tendency for substance P to decrease in concentration in eczematous ears versus controls. There was no difference in serotonin concentration, or in the number of serotonin positive mast cells, between the inflamed and control skin, whereas semiquantification of serotonin positive platelets showed an increase in the inflamed (++) compared with control ears (+). Our results indicate that 24 hr after being challenged with the antigen, at the peak of murine skin inflammation, axonal growth, sensory neuropeptides, as well as serotonin may be involved.
引用
收藏
页码:67 / 76
页数:10
相关论文
共 19 条
[1]  
AMEISEN JC, 1989, J IMMUNOL, V142, P3171
[2]   A ROLE FOR PLATELET-RELEASE OF SEROTONIN IN THE INITIATION OF CONTACT SENSITIVITY [J].
ASKENASE, PW ;
GEBA, GP ;
LEVIN, J ;
RATZLAFF, RE ;
ANDERSON, GM ;
USHIO, H ;
PTAK, W ;
MATSUDA, H .
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, 1995, 107 (1-3) :145-147
[3]   ON THE ACCURATE DETERMINATION OF SEROTONIN IN HUMAN PLASMA [J].
BECK, O ;
WALLEN, NH ;
BROIJERSEN, A ;
LARSSON, PT ;
HJEMDAHL, P .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1993, 196 (01) :260-266
[4]   TACHYKININ MULTIPLICITY IN RAT CENTRAL-NERVOUS-SYSTEM AS STUDIED USING ANTISERA RAISED AGAINST SUBSTANCE-P AND NEUROKININ-A [J].
BRODIN, E ;
LINDEFORS, N ;
DALSGAARD, CJ ;
THEODORSSONNORHEIM, E ;
ROSELL, S .
REGULATORY PEPTIDES, 1986, 13 (3-4) :253-272
[5]   Galanin expression in a murine model of allergic contact dermatitis [J].
El-Nour, H ;
Lundeberg, L ;
Boman, A ;
Theodorsson, E ;
Hökfelt, T ;
Nordlind, K .
ACTA DERMATO-VENEREOLOGICA, 2004, 84 (06) :428-432
[6]   SUBSTANCE-P AND CALCITONIN-GENE-RELATED PEPTIDE MODULATE LEUKOCYTE INFILTRATION TO MOUSE SKIN DURING ALLERGIC CONTACT-DERMATITIS [J].
GOEBELER, M ;
HENSELEIT, U ;
ROTH, J ;
SORG, C .
ARCHIVES OF DERMATOLOGICAL RESEARCH, 1994, 286 (06) :341-346
[7]   NEUROPEPTIDES ENHANCE IRRITANT AND ALLERGIC CONTACT-DERMATITIS [J].
GUTWALD, J ;
GOEBELER, M ;
SORG, C .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1991, 96 (05) :695-698
[8]   IMMUNOPEROXIDASE AND ENZYME-HISTOCHEMICAL DEMONSTRATION OF HUMAN-SKIN TRYPTASE IN CUTANEOUS MAST-CELLS IN NORMAL AND MASTOCYTOMA SKIN [J].
HARVIMA, IT ;
NAUKKARINEN, A ;
HARVIMA, RJ ;
FRAKI, JE .
ARCHIVES OF DERMATOLOGICAL RESEARCH, 1988, 280 (06) :363-370
[9]   Multiple messengers in descending serotonin neurons:: localization and functional implications [J].
Hökfelt, T ;
Arvidsson, U ;
Cullheim, S ;
Millhorn, D ;
Nicholas, AP ;
Pieribone, V ;
Seroogy, K ;
Ulfhake, B .
JOURNAL OF CHEMICAL NEUROANATOMY, 2000, 18 (1-2) :75-86
[10]   SEROTONIN UP-REGULATES MITOGEN-STIMULATED B-LYMPHOCYTE PROLIFERATION THROUGH 5-HT1A RECEPTORS [J].
IKEN, K ;
CHHENG, S ;
FARGIN, A ;
GOULET, AC ;
KOUASSI, E .
CELLULAR IMMUNOLOGY, 1995, 163 (01) :1-9