Zebrafish sp7:EGFP: A Transgenic for Studying Otic Vesicle Formation, Skeletogenesis, and Bone Regeneration

被引：123

作者：

DeLaurier, April ^{[1
]}

Eames, B. Frank ^{[1
]}

Blanco-Sanchez, Bernardo ^{[1
]}

Peng, Gang ^{[1
]}

He, Xinjun ^{[1
]}

Swartz, Mary E. ^{[1
]}

Ullmann, Bonnie ^{[1
]}

Westerfield, Monte ^{[1
]}

Kimmel, Charles B. ^{[1
]}

机构：

[1] Univ Oregon, Inst Neurosci, Eugene, OR 97403 USA

来源：

GENESIS | 2010年 / 48卷 / 08期

关键词：

bone; Danio rerio; gene transfer techniques; otic vesicle; regeneration; sp7; OSTEOBLAST DIFFERENTIATION; EXPRESSION; MORPHOGENESIS; OSTERIX; SYSTEM; ACID;

D O I：

10.1002/dvg.20639

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We report the expression pattern and construction of a transgenic zebrafish line for a transcription factor involved in otic vesicle formation and skeletogenesis. The zinc finger transcription factor sp7 (formerly called osterix) is reported as a marker of osteoblasts. Using bacterial artificial chromosome (BAC)-mediated transgenesis, we generated a zebrafish transgenic line for studying skeletal development, Tg(sp7:EGFP)b1212. Using a zebrafish BAC, EGFP was introduced downstream of the regulatory regions of sp7 and injected into one cell-stage embryos. In this transgenic line, GFP expression reproduces endogenous sp7 gene expression in the otic placode and vesicle, and in forming skeletal structures. GFP-positive cells were also detected in adult fish, and were found associated with regenerating fin rays postamputation. This line provides an essential tool for the further study of zebrafish otic vesicle formation and the development and regeneration of the skeleton. genesis 48:505-511,2010. (C) 2010 Wiley-Liss, Inc.

引用

页码：505 / 511

页数：7

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