cDNA-Derived amino acid sequence from rat brain A2aR possesses conserved motifs PMNYM of TM 5 domain, which may be involved in dimerization of A2aR

被引：0

作者：

Luthra, Pratibha Mehta ^{[1
]}

Barodial, Sandeep Kumar ^{[1
]}

Prakash, Amresh ^{[1
]}

Ramraghubir ^{[2
]}

机构：

[1] Univ Delhi, Dr BR Ambedkar Ctr Biomed Res, POB 2148, Delhi 110007, India

[2] Cent Drug Res Inst, Dept Pharmacol, Lucknow 226001, Uttar Pradesh, India

来源：

PATTERN RECOGNITION IN BIOINFORMATICS, PROCEEDINGS | 2007年 / 4774卷

关键词：

adenosine A(2a) receptor; cDNA; GPCRs; RT-PCR; TM; 5;

D O I：

暂无

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The human adenosine A(2a) receptor (A(2a)R) belongs to the family of G-protein coupled receptors (GPCRs), characterized by seven transmembrane (TM) helices. TMs are involved in various cellular processes including dimerization-mediated recognition of ligand. TM5 has been suggested to self associate and may be involved in the dimerization of A(2a)R. However the role of dimerization and the motifs involved in dimerization of TM 5 have not been revealed. To study the folding and assembly of A2aR, the cDNA of the adenosine A2aR from rat brain was isolated and sequenced (DQ098650). The computational analysis (gil70727927lgblAAZ07991.1l) showed that the protein of 42 amino acid residues aligned in TM 5 domain region of AA2AR_RAT (P30543). PROSITE search illustrated that the motif PMNYM was conserved in A2,R and the motif PMSYM was present in A(2b)R respectively. The minimal dimerization motif in the TM 5 domain of the rat A2a receptor sequence DQ098650 has found to be the motif PXXXM/Y.

引用

页码：41 / 50

页数：10