Coupling Peptide Antigens to Virus-Like Particles or to Protein Carriers Influences the Th1/Th2 Polarity of the Resulting Immune Response

被引：17

作者：

Pomwised, Rattanaruji ^{[1
]}

Intamaso, Uraiwan ^{[2
]}

Teintze, Martin ^{[3
]}

Young, Mark ^{[4
]}

Pincus, Seth H. ^{[5
]}

机构：

[1] Prince Songkla Univ, Sch Med, Dept Microbiol, Hat Yai 90110, Thailand

[2] Burapha Univ, Fac Allied Hlth Sci, Chon Buri 20131, Thailand

[3] Montana State Univ, Dept Chem & Biochem, Bozeman, MT 59717 USA

[4] Montana State Univ, Dept Plant Sci, Bozeman, MT 59717 USA

[5] Louisianna State Univ, Sch Med, Dept Pediat & Microbiol, New Orleans, LA 70118 USA

来源：

VACCINES | 2016年 / 4卷 / 02期

关键词：

group B streptococcus; Streptococcus agalactiae; capsular polysaccharide; antigen; mimotope; synthetic peptide; COWPEA MOSAIC-VIRUS; CHIMERAS; EXPRESSION; MUTANT; CELLS;

D O I：

10.3390/vaccines4020015

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We have conjugated the S9 peptide, a mimic of the group B streptococcal type III capsular polysaccharide, to different carriers in an effort to elicit an optimal immune response. As carriers, we utilized the soluble protein keyhole limpet hemocyanin and virus-like particles (VLPs) from two plant viruses, Cowpea Chlorotic Mottle Virus and Cowpea Mosaic Virus. We have found that coupling the peptide to the soluble protein elicits a Th2 immune response, as evidenced by the production of the peptide-specific IgG1 antibody and IL-4/IL-10 production in response to antigen stimulation, whereas the peptide conjugated to VLPs elicited a Th1 response (IgG2a, IFN-). Because the VLPs used as carriers package RNA during the assembly process, we hypothesize that this effect may result from the presence of nucleic acid in the immunogen, which affects the Th1/Th2 polarity of the response.

引用

页数：10

共 19 条

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