Expression and antiviral activity of a β-defensin-like peptide identified in the rainbow trout (Oncorhynchus mykiss) EST sequences

被引：102
作者：
Falco, A.
Chico, V.
Marroqui, L.
Perez, L.
Coll, J. M.
Estepa, A. [1 ]
机构：
[1] Miguel Hernandez Univ, IBMC, Elche 03202, Spain
[2] INIA, SIGT, Madrid 28040, Spain
来源：
MOLECULAR IMMUNOLOGY | 2008年 / 45卷 / 03期
关键词：
antimicrobial peptide; defensin; VHSV; IFN; Mx;
D O I：
10.1016/j.molimm.2007.06.358
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The in silico identification of a P-defensin-like peptide sequence (omBD-1) in the rainbow trout (Oncorhynchuss mykiss) database of salmonid EST is reported here. We have studied the transcript expression of this P-defensin-like sequence in different organs and expressed the recombinant peptide in a fish cell line. Finally, we have demonstrated the in vitro antiviral activity of the recombinant trout P-defensin-like peptide against viral haemorrhagic septicaernia rhabdovirus (VHSV), one of the most devastating viruses for worldwide aquaculture. Thus, the resistance to VHSV infection of EPC cells transfected with pMCV 1.4-omBD-1 has been shown. Since EPC cells transfected with omBD-1 produced acid and heat stable antiviral activity and up regulation of Mx, a type IIFN-mediated mechanism of antiviral action is suggested. To our knowledge, this is the first report showing biological activity of a P-defensin-like peptide from any fish. (c) 2007 Elsevier Ltd. All rights reserved.
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收藏
页码：757 / 765
页数：9
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