A comparison of methods for purification and concentration of norovirus GII-4 capsid virus-like particles

被引：77

作者：

Huhti, L. ^{[1
]}

Blazevic, V. ^{[1
]}

Nurminen, K. ^{[1
]}

Koho, T. ^{[2
]}

Hytonen, V. P. ^{[2
]}

Vesikari, T. ^{[1
,3
]}

机构：

[1] Univ Tampere, Sch Med, Vaccine Res Ctr, Tampere 33520, Finland

[2] Univ Tampere, Tampere Univ Hosp, Inst Med Technol, Tampere 33520, Finland

[3] Tampere Univ Hosp, Dept Pediat, Tampere, Finland

来源：

ARCHIVES OF VIROLOGY | 2010年 / 155卷 / 11期

关键词：

NORWALK VIRUS; IMMUNE-RESPONSES; VOLUNTEERS; STABILITY; ANTIGENS; BINDING; PROTEIN; MICE;

D O I：

10.1007/s00705-010-0768-z

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Noroviruses (NoVs) are one of the leading causes of acute gastroenteritis worldwide. NoV GII-4 VP1 protein was expressed in a recombinant baculovirus system using Sf9 insect cells. Several methods for purification and concentration of virus-like particles (VLPs) were evaluated. Electron microscopy (EM) and histo-blood group antigen (HBGA) binding assays showed that repeated sucrose gradient purification followed by ultrafiltration resulted in intact VLPs with excellent binding to H type 3 antigens. VLPs were stable for at least 12 months at 4 degrees C, and up to 7 days at ambient temperature. These findings indicate that this method yielded stable and high-quality VLPs.

引用

页码：1855 / 1858

页数：4

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