Composition and Functions of the Gut Microbiome in Pediatric Obesity: Relationships with Markers of Insulin Resistance

被引：27

作者：

Orsso, Camila E. ^{[1
]}

Peng, Ye ^{[2
]}

Deehan, Edward C. ^{[3
,4
]}

Tan, Qiming ^{[4
]}

Field, Catherine J. ^{[5
]}

Madsen, Karen L. ^{[3
]}

Walter, Jens ^{[6
,7
]}

Prado, Carla M. ^{[1
]}

Tun, Hein M. ^{[2
]}

Haqq, Andrea M. ^{[4
,5
]}

机构：

[1] Univ Alberta, Li Ka Shing Ctr Hlth Innovat 4 002, Dept Agr Food & Nutr Sci, Human Nutr Res Unit, Edmonton, AB T6G 2E1, Canada

[2] Univ Hong Kong, Sch Publ Hlth, HKU Pasteur Res Pole, Hong Kong 999077, Peoples R China

[3] Univ Alberta, Dept Med, Edmonton, AB T6G 2C2, Canada

[4] Univ Alberta, Dept Pediat, Edmonton, AB T6G 2R3, Canada

[5] Univ Alberta, Dept Agr Food & Nutr Sci, Edmonton, AB T6G 2E1, Canada

[6] Natl Univ Ireland, Univ Coll Cork, APC Microbiome Ireland, Sch Microbiol, Cork T12 YT20, Ireland

[7] Natl Univ Ireland, Univ Coll Cork, Dept Med, Cork T12 YT20, Ireland

来源：

MICROORGANISMS | 2021年 / 9卷 / 07期

基金：

加拿大健康研究院;

关键词：

gut microbiome; microbiota; shotgun metagenomics; insulin resistance; HOMA-IR; childhood obesity; HOMEOSTASIS MODEL ASSESSMENT; CARBOHYDRATE-ACTIVE ENZYMES; CHAIN FATTY-ACIDS; METABOLIC SYNDROME; EARLY-ONSET; ADOLESCENT OBESITY; CHILDHOOD OBESITY; PHYSICAL-ACTIVITY; WEIGHT STATUS; CHILDREN;

D O I：

10.3390/microorganisms9071490

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The gut microbiome is hypothesized to play a crucial role in the development of obesity and insulin resistance (IR); the pathways linking the microbiome to IR in pediatrics have yet to be precisely characterized. We aimed to determine the relationship between the gut microbiome composition and metabolic functions and IR in children with obesity. In a cross-sectional study, fecal samples from children with obesity (10-16 years old) were collected for taxonomical and functional analysis of the fecal microbiome using shotgun metagenomics. The homeostatic model assessment for insulin resistance (HOMA-IR) was determined using fasting glucose and insulin. Associations between HOMA-IR and alpha-diversity measures as well as metabolic pathways were evaluated using Spearman correlations; relationships between HOMA-IR and beta-diversity were assessed by permutational multivariate analysis of variance. Twenty-one children (nine males; median: age = 12.0 years; BMI z-score = 2.9; HOMA-IR = 3.6) completed the study. HOMA-IR was significantly associated with measures of alpha-diversity but not with beta-diversity. Children with higher HOMA-IR exhibited lower overall species richness, Firmicutes species richness, and overall Proteobacteria species Shannon diversity. Furthermore, HOMA-IR was inversely correlated with the abundance of pathways related to the biosynthesis of lipopolysaccharides, amino acids, and short-chain fatty acids, whereas positive correlations between HOMA-IR and the peptidoglycan biosynthesis pathways were observed. In conclusion, insulin resistance was associated with decreased microbial alpha-diversity measures and abundance of genes related to the metabolic pathways. Our study provides a framework for understanding the microbial alterations in pediatric obesity.

引用

页数：18

共 100 条

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