Entactin expression by rat lung and rat alveolar epithelial cells

被引:31
|
作者
Senior, RM
Griffin, GL
Mudd, MS
Moxley, MA
Longmore, WJ
Pierce, RA
机构
[1] UNIV WASHINGTON,DEPT MED,JEWISH HOSP ST LOUIS,SCH MED,DIV DERMATOL,ST LOUIS,MO 63110
[2] ST LOUIS UNIV,SCH MED,DOISEY DEPT BIOCHEM & MOLEC BIOL,ST LOUIS,MO
关键词
D O I
10.1165/ajrcmb.14.3.8845174
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Although the composition of the subepithelial basement membrane of the alveolar septum has been studied in detail, there is relatively little information about which cells produce it, We examined intact rat lung and isolated rat alveolar type II cells for the expression of entactin, an integral basement membrane component that binds laminin and type IV collagen. By Northern analysis, late gestation and early neonatal rat lungs expressed high levels of entactin mRNA whereas lungs from adult animals had only minimal levels of entactin mRNA. These latter findings were confirmed by in situ hybridization, which showed prominent signal for entactin mRNA in cells in the alveolar walls of neonatal animals and no signal for entactin mRNA in the alveolar walls of lungs from adult animals. The entactin mRNA throughout the alveolar walls of neonatal animals was not limited to cells that expressed surfactant-associated protein C mRNA, a marker of alveolar type II cells. Freshly harvested adult alveolar type II cells and alveolar type II cells in culture for < 6 days expressed none to minimal entactin mRNA or protein. However, with longer periods in culture, both entactin mRNA and entactin protein synthesis were evident and progressively increased. In situ hybridization indicated that > 60 % of the alveolar epithelial cells expressed entactin mRNA with increasing time in culture. When cultured on Engelbreth-Holm-Swarm matrix, alveolar type II cells showed the same time course of entactin mRNA expression as cells cultured on plastic. Neonatal lung mesenchymal cells produced abundant entactin in culture, consistent with the likelihood that these cells are the principal source of entactin in alveolar walls in the developing lung. These results indicate that entactin production in the normal alveolar wall occurs primarily during lung development and that mesenchymal cells are probably the principal source of production, However, because adult alveolar epithelial cells synthesize entactin in culture, it is possible that alveolar epithelium contributes to the entactin in the alveolar subepithelial basement membrane.
引用
收藏
页码:239 / 247
页数:9
相关论文
共 50 条
  • [32] Rat synexin: cDNA sequence and mRNA expression in lung and alveolar type II cells
    Raha, A
    Spitzer, AR
    Chander, A
    FASEB JOURNAL, 2001, 15 (04): : A494 - A494
  • [33] EFFECTS OF HISTONE MODIFICATION ON CHEMOKINE EXPRESSION IN ALVEOLAR EPITHELIAL TYPE CELLS IN A RAT MODEL OF COPD
    Gan, L.
    Li, C.
    Xu, W.
    Guo, X.
    RESPIROLOGY, 2011, 16 : 119 - 119
  • [34] FIBRIN DEGRADATION BY RAT PULMONARY ALVEOLAR EPITHELIAL-CELLS
    SIMON, RH
    GROSS, TJ
    EDWARDS, JA
    SITRIN, RG
    AMERICAN JOURNAL OF PHYSIOLOGY, 1992, 262 (04): : L482 - L488
  • [35] Rat alveolar epithelial type I cells express CFTR
    Johnson, M
    Jain, L
    Eaton, D
    Dobbs, L
    FASEB JOURNAL, 2006, 20 (04): : A746 - A746
  • [36] DIFFERENTIATION-RELATED EXPRESSION OF ICAM-1 BY RAT ALVEOLAR EPITHELIAL-CELLS
    CHRISTENSEN, PJ
    KIM, S
    SIMON, RH
    TOEWS, GB
    PAINE, R
    AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 1993, 8 (01) : 9 - 15
  • [37] Lipopolysaccharide induces functional ICAM-1 expression in rat alveolar epithelial cells in vitro
    Madjdpour, C
    Oertli, B
    Ziegler, U
    Bonvini, JM
    Pasch, T
    Beck-Schimmer, B
    AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 2000, 278 (03) : L572 - L579
  • [38] Regulation of lung fibroblast tropoelastin expression by alveolar epithelial cells
    Mariani, TJ
    Dunsmore, SE
    Li, QL
    Ye, XM
    Pierce, RA
    AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 1998, 274 (01) : L47 - L57
  • [39] Modulation of Na+ transport and epithelial sodium channel expression by protein kinase C in rat alveolar epithelial cells
    Yamagata, T
    Yamagata, Y
    Massé, C
    Tessier, MC
    Brochiero, E
    Dagenais, A
    Berthiaume, Y
    CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, 2005, 83 (11) : 977 - 987
  • [40] MACROPHAGE INFLAMMATORY PROTEIN-1 AND PROTEIN-2 - EXPRESSION BY RAT ALVEOLAR MACROPHAGES, FIBROBLASTS, AND EPITHELIAL-CELLS AND IN RAT LUNG AFTER MINERAL DUST EXPOSURE
    DRISCOLL, KE
    HASSENBEIN, DG
    CARTER, J
    POYNTER, J
    ASQUITH, TN
    GRANT, RA
    WHITTEN, J
    PURDON, MP
    TAKIGIKU, R
    AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 1993, 8 (03) : 311 - 318