Crystallization and X-ray analysis of NH3-dependent NAD+ synthetase from Helicobacter pylori

被引：1
|
作者：
Kang, GB [1 ]
Kim, YS [1 ]
Im, YJ [1 ]
Rho, SH [1 ]
Eom, SH [1 ]
机构：
[1] Kwanju Inst Sci & Technol, Dept Life Sci, Gwangju 500712, South Korea
来源：
PROTEIN AND PEPTIDE LETTERS | 2003年 / 10卷 / 04期
关键词：
amidotransferase; Helicobacter pylori; crystallization; NAD(+) synthetase;
D O I：
10.2174/0929866033478843
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The ubiquitous NAD(+) synthetase catalyzes the key step in the biosynthesis of nicotinamide adenine dinucleotide. NH3-dependent NAD(+) synthetase from Helicobacter pylori was purified to homogeneity and crystallized using PEG 1500 as a preciptant. The crystal diffracted up to a resolution of 2.3+ and was found to belong to space group C2 with unit cell dimensions of a = 93.8, b = 48.3, c = 64.2 A and alpha = gamma = 90, beta = 110.0degrees.
引用
收藏
页码：418 / 421
页数：4
相关论文
共 50 条
  • [1] Structural Analysis of the NH3-dependent NAD+ Synthetase from Deinococcus radiodurans
    Park, Young Woo
    Yeo, Hyun Ku
    Lee, Jae Young
    BULLETIN OF THE KOREAN CHEMICAL SOCIETY, 2014, 35 (09): : 2863 - 2866
  • [2] Crystal structure of NH3-dependent NAD(+) synthetase from Bacillus subtilis
    Rizzi, M
    Nessi, C
    Mattevi, A
    Coda, A
    Bolognesi, M
    Galizzi, A
    EMBO JOURNAL, 1996, 15 (19) : 5125 - 5134
  • [3] Structural and molecular dynamics of ammonia transport in Staphylococcus aureus NH3-dependent NAD synthetase
    Sultana, Kazi Nasrin
    Srivastava, Sandeep Kumar
    INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2022, 203 : 593 - 600
  • [4] Crystallization and preliminary X-ray crystallographic analysis of orotate phosphoribosyltransferase from Helicobacter pylori
    Kim, MK
    Song, HE
    Kim, YS
    Rho, SH
    Im, YJ
    Lee, JH
    Kang, GB
    Eom, SH
    MOLECULES AND CELLS, 2003, 15 (03) : 361 - 363
  • [5] Crystallization and X-ray data collection of HP0902 from Helicobacter pylori 26695
    Sim, Dae-Won
    Song, Jung Hyun
    Lee, Woo Cheol
    Lee, Yoo-Sup
    Kim, Hye-Yeon
    Won, Hyung-Sik
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2011, 67 : 1619 - 1622
  • [6] Crystallization and X-ray analysis of the extracellular adhesion domain of Helicobacter pylori adhesin A: the significance of the cation composition in the crystallization precipitant
    Guo, Ling
    Zhang, Jinyong
    Cui, Liwei
    Liu, Dong
    Ma, Bo
    Wang, Shufeng
    Li, Haibo
    Wu, Yuzhang
    Liu, Wei
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2017, 73 : 202 - 208
  • [7] Overexpression, purification, crystallization, and preliminary X-ray characterization of a methionine sulfoxide reductase AB from Helicobacter pylori
    Kitaik Lee
    Hyun Sook Kim
    Won Kyu Lee
    Ah Reum Han
    Jun Soo Kim
    Kwang Yeon Hwang
    Journal of the Korean Society for Applied Biological Chemistry, 2014, 57 : 23 - 26
  • [8] Overexpression, Purification, Crystallization, and Preliminary X-ray Characterization of a Methionine Sulfoxide Reductase AB from Helicobacter pylori
    Lee, Kitaik
    Kim, Hyun Sook
    Lee, Won Kyu
    Han, Ah Reum
    Kim, Jun Soo
    Hwang, Kwang Yeon
    JOURNAL OF THE KOREAN SOCIETY FOR APPLIED BIOLOGICAL CHEMISTRY, 2014, 57 (01): : 23 - 26
  • [9] Cloning, expression, purification, crystallization and preliminary X-ray diffraction studies of NAD synthetase from methicillin-resistant Staphylococcus aureus
    Arbade, Gajanan Kashinathrao
    Srivastava, Sandeep Kumar
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2015, 71 : 763 - 769
  • [10] Crystallization and preliminary X-ray diffraction analysis of arginyl-tRNA synthetase from Escherichia coli
    Zhou, M
    Wang, ED
    Campbell, RL
    Wang, YL
    Lin, SX
    PROTEIN SCIENCE, 1997, 6 (12) : 2636 - 2638
12345