Although, various analyses have concerned an effect of various kind of biodiesel on engine, in this study, biodiesel production from Safflower oil through transesterification. methanol to oil (M: O) molar ratio, catalyst concentration, reaction temperature, time and stirring rate have been chosen to investigate their effect on biodiesel yield. The optimum conditions for 90% biodiesel yield have been achieved at M:O molar ratio of 16:1, 4 g of potassium hydroxide (KOH), Temperature of 60 degrees C, time of 4 h and stirring rate of 700 rpm. Different blends (B20, B30, and B40) of safflower biodiesel were prepared and investigate the engine performance and emissions when employing Safflower biodiesel and its blends. However, the outcome from the study depicted that BTE of B20 shows 33.63% which is closer to diesel (34.63%). The emissions CO, HC, smoke were considerably reduced using SAF biodiesel blended fuels than diesel fuel. Alongside the NOx emission has increased due to higher cetane number of SAF biodiesel fuels. (c) 2021 Elsevier Ltd. All rights reserved. Selection and peer-review under responsibility of the scientific committee of the 3rd International Conference on Materials, Manufacturing and Modelling.