Methods of Glioma Sample Processing for Molecular Diagnosis for the Glioma Tissue Bank Project

被引：4

作者：

Shi, Zhi-feng ^{[1
]}

Aibaidula, Abudumijiti ^{[1
]}

Tang, Qi-sheng ^{[1
]}

Shen, Yi-wen ^{[1
]}

Chen, Hong ^{[2
]}

Wu, Jin-song ^{[1
]}

Qin, Zhi-yong ^{[1
]}

Zhu, Jian-hong ^{[1
]}

Mao, Ying ^{[1
]}

Zhou, Liang-fu ^{[1
]}

机构：

[1] Fudan Univ, Shanghai Med Coll, Huashan Hosp, Glioma Surg Div,Neurol Surg Dept, Shanghai 200040, Peoples R China

[2] Fudan Univ, Shanghai Med Coll, Huashan Hosp, Dept Neuropathol, Shanghai 200040, Peoples R China

来源：

BIOPRESERVATION AND BIOBANKING | 2015年 / 13卷 / 01期

关键词：

RESPONSE ASSESSMENT; DNA EXTRACTION; NEUROONCOLOGY;

D O I：

10.1089/bio.2014.0089

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Genome-wide sequencing in glioma samples provides comprehensive insights into oncogenesis and malignant transformation. Several distinct biomarkers have been proven to have clinical significance and are being widely applied in routine clinical practice. Standard sample processing lays the foundation for successful molecular testing. In this study, we found intraoperative neuronavigation ensured higher tumor purity during sample collection, and an automated device helped improve DNA quality and increased yields. These two technologies are beneficial for glioma tissue bank construction and provide for accurate molecular testing during routine clinical practice.

引用

页码：31 / 36

页数：6

共 13 条

[1]

Aibaidula A, 2014, CELL TISSUE BANK

[2] Comprehensive genomic characterization defines human glioblastoma genes and core pathways [J].

Chin, L. ;

Meyerson, M. ;

Aldape, K. ;

Bigner, D. ;

Mikkelsen, T. ;

VandenBerg, S. ;

Kahn, A. ;

Penny, R. ;

Ferguson, M. L. ;

Gerhard, D. S. ;

Getz, G. ;

Brennan, C. ;

Taylor, B. S. ;

Winckler, W. ;

Park, P. ;

Ladanyi, M. ;

Hoadley, K. A. ;

Verhaak, R. G. W. ;

Hayes, D. N. ;

Spellman, Paul T. ;

Absher, D. ;

Weir, B. A. ;

Ding, L. ;

Wheeler, D. ;

Lawrence, M. S. ;

Cibulskis, K. ;

Mardis, E. ;

Zhang, Jinghui ;

Wilson, R. K. ;

Donehower, L. ;

Wheeler, D. A. ;

Purdom, E. ;

Wallis, J. ;

Laird, P. W. ;

Herman, J. G. ;

Schuebel, K. E. ;

Weisenberger, D. J. ;

Baylin, S. B. ;

Schultz, N. ;

Yao, Jun ;

Wiedemeyer, R. ;

Weinstein, J. ;

Sander, C. ;

Gibbs, R. A. ;

Gray, J. ;

Kucherlapati, R. ;

Lander, E. S. ;

Myers, R. M. ;

Perou, C. M. ;

McLendon, Roger .

NATURE, 2008, 455 (7216) :1061-1068

[3] Application of the NucliSENS easyMAG system for nucleic acid extraction: optimization of DNA extraction for molecular diagnosis of parasitic and fungal diseases [J].

Jeddi, Fakhri ;

Piarroux, Renaud ;

Mary, Charles .

PARASITE, 2013, 20

[4]

Mannu C, 2014, LEUKEMIA LYMPHOMA, P1

[5] A stabilizing reagent prevents cell-free DNA contamination by cellular DNA in plasma during blood sample storage and shipping as determined by digital PCR [J].

Norton, S. E. ;

Lechner, J. M. ;

Williams, T. ;

Fernando, M. R. .

CLINICAL BIOCHEMISTRY, 2013, 46 (15) :1561-1565

[6] Comparative study of DNA extraction methodologies from goat sperm and its effects on polymerase chain reaction analysis [J].

Silva, E. C. B. ;

Pelinca, M. A. ;

Acosta, A. C. ;

Silva, D. M. F. ;

Gomes Filho, M. A. ;

Guerra, M. M. P. .

GENETICS AND MOLECULAR RESEARCH, 2014, 13 (03) :6070-6078

[7] High-grade malignant glioma: ESMO Clinical Practice Guidelines for diagnosis, treatment and follow-up [J].

Stupp, R. ;

Tonn, J. -C. ;

Brada, M. ;

Pentheroudakis, G. .

ANNALS OF ONCOLOGY, 2010, 21 :v190-v193

[8] Multigene profiling to identify alternative treatment options for glioblastoma: a pilot study [J].

Tabone, Tania ;

Abuhusain, Hazem J. ;

Nowak, Anna K. ;

Erber, Wendy N. ;

McDonald, Kerrie L. .

JOURNAL OF CLINICAL PATHOLOGY, 2014, 67 (07) :550-555

[9] Response assessment in neuro-oncology (a report of the RANO group): assessment of outcome in trials of diffuse low-grade gliomas [J].

van den Bent, M. J. ;

Wefel, J. S. ;

Schiff, D. ;

Taphoorn, M. J. B. ;

Jaeckle, K. ;

Junck, L. ;

Armstrong, T. ;

Choucair, A. ;

Waldman, A. D. ;

Gorlia, T. ;

Chamberlain, M. ;

Baumert, B. G. ;

Vogelbaum, M. A. ;

Macdonald, D. R. ;

Reardon, D. A. ;

Wen, P. Y. ;

Chang, S. M. ;

Jacobs, A. H. .

LANCET ONCOLOGY, 2011, 12 (06) :583-593

[10] Comparison of automated nucleic acid extraction methods for the detection of cytomegalovirus DNA in fluids and tissues [J].

Waggoner, Jesse J. ;

Pinsky, Benjamin A. .

PEERJ, 2014, 2

← 1 2 →