Effectiveness of polyclonal and monoclonal antibodies prepared for an immunoassay of the etofenprox insecticide

被引：19
|
作者：
Miyake, S
Hayashi, A
Kumeta, T
Kitajima, K
Kita, H
Ohkawa, H
机构：
[1] Kobe Univ, Fac Agr, Dept Biol & Environm Sci, Nada Ku, Kobe, Hyogo 6570013, Japan
[2] Iatron Labs Inc, Cent Res Inst, Chiba 2892247, Japan
[3] Mitsui Toatsu Chem Co Ltd, Inst Life Sci, Chiba 2970017, Japan
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1998年 / 62卷 / 05期
关键词：
polyclonal antibody; monoclonal antibody; immunoassay; etofenprox; insecticide;
D O I：
10.1271/bbb.62.1001
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Two polyclonal antibodies and three monoclonal antibodies specific to the etofenprox insecticide were prepared from rabbits and mice, respectively. The monoclonal antibodies were more reactive with etofenprox than the polyclonal antibodies by C-ELISA. Monoclonal antibody cl 205-65 was found to be the most tolerant to methanol, most highly reactive and most specific to etofenprox among the antibodies tested.
引用
收藏
页码：1001 / 1004
页数：4
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