Thermodynamic analysis of the interaction of ethidium bromide with tetradeoxyribonucleotide 5'-d(GpCpGpC) using H-1-NMR data

被引：0

作者：

Veselkov, AN ^{[1
]}

Dymant, LN ^{[1
]}

Bolotin, PA ^{[1
]}

Baranovskii, SF ^{[1
]}

Veselkov, DA ^{[1
]}

Shipp, D ^{[1
]}

Davies, D ^{[1
]}

机构：

[1] UNIV LONDON BIRKBECK COLL,LONDON MC1H 0PP,ENGLAND

来源：

MOLECULAR BIOLOGY | 1996年 / 30卷 / 01期

关键词：

thermodynamic parameters; intercalation; ethidium bromide;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Temperature dependences of proton chemical shifts for ethidium bromide in aqueous solution with tetradeoxyribonucleotide 5'-d(GpCpGpC) were studied by 500-MHz pulse NMR spectrometry. Calculation of the thermodynamic parameters of complexation yielded estimates for contributions of reactions giving rise to different types of complexes under complex equilibrium conditions. Gibbs energies, enthalpies, and entropies were determined for the formation of 1:1, 2:1, 1:2, and 2:2 complexes between EthBr and GCGC.

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页码：111 / 116

页数：6

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