Production and characterization of PHB from a novel isolate Comamonas sp from a dairy effluent sample and its application in cell culture

被引：25

作者：

Prabisha, Thunoli Payyanvalappil ^{[1
]}

Sindhu, Raveendran ^{[1
]}

Binod, Parameswaran ^{[1
]}

Sankar, Vandana ^{[2
]}

Raghu, Kozhiparambil Gopalan ^{[2
]}

Pandey, Ashok ^{[1
]}

机构：

[1] CSIR, Div Biotechnol, Natl Inst Interdisciplinary Sci & Technol, Trivandrum 695019, Kerala, India

[2] CSIR, Agroproc Div, Natl Inst Interdisciplinary Sci & Technol, Trivandrum 695019, Kerala, India

来源：

BIOCHEMICAL ENGINEERING JOURNAL | 2015年 / 101卷

关键词：

Poly-3-hydroxybutyrate; Comamonas sp; Fermentation; Cell culture; Matrices; Optimization; POLY; 3-HYDROXYBUTYRATE; POLYHYDROXYALKANOATES; POLY-3-HYDROXYBUTYRATE; GLYCEROL;

D O I：

10.1016/j.bej.2015.05.012

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In the present study, media engineering was carried out to optimize various process parameters affecting fermentation for PHB production by Comamonas sp. The optimum conditions for PHB production were yeast extract concentration of 1%, pH 6.0 and inoculum concentration of 5.5%. Under optimized conditions the PHB yield was 0.523 g/g. The extracted polymer was characterized by FTIR and H-1 NMR. Nanomatrices and solid walled matrices were prepared using the extracted PHB. Cytotoxic studies revealed that both solid walled and nanomatrices prepared from the extracted PHB showed almost 100% survivals of H9c2 cells. This is the first report on application of extracted PHB from Comamonas sp. as an extracellular matrix for cell culture. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：150 / 159

页数：10

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