Live imaging and quantitation of nascent transcription using the MS2/MCP system in the Drosophila embryo

被引：10

作者：

Hoppe, Caroline ^{[1
]}

Ashe, Hilary L. ^{[1
]}

机构：

[1] Univ Manchester, Fac Biol Med & Hlth, Manchester M13 9PT, England

来源：

STAR PROTOCOLS | 2021年 / 2卷 / 01期

基金：

英国惠康基金;

关键词：

SINGLE CELLS; EXPRESSION;

D O I：

10.1016/j.xpro.2021.100379

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Visualizing transcription live in Drosophila is providing important new insights into the spatiotemporal regulation of transcription. Here, we describe a protocol to visualize and quantitate transcription from gene loci that are tagged with MS2 stem-loop sequences in the Drosophila embryo. MS2 stem-loop sequences are recognized by a coat protein fused to a fluorescent protein and visualized with microscopy. We also describe an analysis pipeline to extract and subsequently quantify transcription dynamics.For complete details on the use and execution of this protocol, please refer to Hoppe et al. (2020).

引用

页数：21

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