Simultaneous time- and wavelength-resolved fluorescence spectroscopy for near real-time tissue diagnosis

被引：45
|
作者：
Sun, Yinghua [1 ,2 ]
Liu, Rui [1 ]
Elson, Daniel S. [3 ]
Hollars, Christopher W. [2 ]
Jo, Javier A. [1 ]
Park, Jesung [1 ]
Sun, Yang [1 ]
Marcu, Laura [1 ,2 ]
机构：
[1] Univ Calif Davis, Dept Biomed Engn, Davis, CA 95616 USA
[2] Univ Calif Davis, NSF, Ctr Biophoton Sci & Technol, Sacramento, CA 95817 USA
[3] Univ London Imperial Coll Sci Technol & Med, Inst Biomed Engn, London SW7 2AZ, England
来源：
OPTICS LETTERS | 2008年 / 33卷 / 06期
关键词：
D O I：
10.1364/OL.33.000630
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
A novel fiber-optic-based method for simultaneous time- and wavelength-resolved fluorescence spectroscopy for the rapid diagnosis of diseased tissue is demonstrated. By combining multiple bandpass and dichroic filters (405/40, 460/50, and 550/50) with different lengths of optical fiber (1, 10, and 19 m) acting as an optical delay this system enables the near real-time acquisition and characterization of time-resolved fluorescence spectra using a single detector and excitation input. The recording of multiple fluorescence response pulses at selected wavelengths can be completed in hundreds of nanoseconds, which provides the capability of a real-time characterization of biological systems. (C) 2008 Optical Society of America.
引用
收藏
页码：630 / 632
页数：3
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