Identification of Noncoding Transcripts from within CENP-A Chromatin at Fission Yeast Centromeres

被引：98
作者：
Choi, Eun Shik [2 ,3 ]
Stralfors, Annelie [1 ]
Castillo, Araceli G. [2 ,3 ]
Durand-Dubief, Mickael [1 ]
Ekwall, Karl [1 ]
Allshire, Robin C. [2 ,3 ]
机构：
[1] Karolinska Inst, Novum, Dept Biosci & Nutr, Ctr Biosci, S-14157 Huddinge, Sweden
[2] Univ Edinburgh, Wellcome Trust Ctr Cell Biol, Edinburgh EH9 3JR, Midlothian, Scotland
[3] Univ Edinburgh, Inst Cell Biol, Edinburgh EH9 3JR, Midlothian, Scotland
来源：
JOURNAL OF BIOLOGICAL CHEMISTRY | 2011年 / 286卷 / 26期
基金：
瑞典研究理事会; 英国惠康基金;
关键词：
COMPREHENSIVE ANALYSIS; EPIGENETIC REGULATION; RNA; HETEROCHROMATIN; PROTEIN; GENE; KINETOCHORES; COMPONENTS; TURNOVER; DYNAMICS;
D O I：
10.1074/jbc.M111.228510
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The histone H3 variant CENP-A is the most favored candidate for an epigenetic mark that specifies the centromere. In fission yeast, adjacent heterochromatin can direct CENP-A(Cnp1) chromatin establishment, but the underlying features governing where CENP-A(Cnp1) chromatin assembles are unknown. We show that, in addition to centromeric regions, a low level of CENP-A(Cnp1) associates with gene promoters where histone H3 is depleted by the activity of the Hrp1(Chd1) chromatin-remodeling factor. Moreover, we demonstrate that noncoding RNAs are transcribed by RNA polymerase II (RNAPII) from CENP-A(Cnp1) chromatin at centromeres. These analyses reveal a similarity between centromeres and a subset of RNAPII genes and suggest a role for remodeling at RNAPII promoters within centromeres that influences the replacement of histone H3 with CENP-A(Cnp1).
引用
收藏
页码：23600 / 23607
页数：8
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