Anti-inflammatory potential of Antrodia Camphorata through inhibition of NOS, COX-2 and cytokines via the NF-κB pathway

被引：161

作者：

Hseu, YC ^{[1
]}

Wu, FY ^{[1
]}

Wu, JJ ^{[1
]}

Chen, JY ^{[1
]}

Chang, WH ^{[1
]}

Lu, FJ ^{[1
]}

Lai, YC ^{[1
]}

Yang, HL ^{[1
]}

机构：

[1] China Med Univ, Inst Nutr, Taichung 40421, Taiwan

来源：

INTERNATIONAL IMMUNOPHARMACOLOGY | 2005年 / 5卷 / 13-14期

关键词：

antrodia camphorata; cytokines; inducible nitric oxide synthase (iNOS); cyclooxygenase 2 (COX-2); nuclear factor-kappa B (NF-kappa B);

D O I：

10.1016/j.intimp.2005.06.013

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Antrodia camphorata (A. camphorata), well known in Taiwan as a traditional Chinese medicine, has been shown to exhibit antioxidant and anticancer effects. In the present study, therefore, we have examined the effects of the fermented culture broth of A. camphorata (25-100 mu g/ml) in terms of lipopolysaccharide (LPS)-induced nitric oxide (NO) and prostaglandin E-2 (PGE(2)) production, and inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein expression in RAW 264.7 macrophages. Our results indicate concentration-dependent A. camphorata inhibition of LPS-induced NO and PGE(2) production, without appreciable cytotoxicity on the RAW 264.7 cells. A. camphorata also attenuates the production of LPS-induced tumor necrosis factor (TNF-alpha) and interleukin (IL)-1 beta. Furthermore, A. camphorata blocks the I kappa B-alpha degradation induced by LPS. These results indicate that A. camphorata inhibits LPS induction of cytokine, iNOS and COX-2 expression by blocking NF-kappa B activation. Therefore, we report the first confirmation of the anti-inflammatory potential of this traditionally employed herbal medicine in vitro. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：1914 / 1925

页数：12

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