Characterization of 12 microsatellite loci for the Pacific lamprey, Entosphenus tridentatus (Petromyzontidae), and cross-amplification in five other lamprey species

被引：11

作者：

Spice, E. K. ^{[1
]}

Whitesel, T. A. ^{[2
]}

McFarlane, C. T. ^{[1
]}

Docker, M. F. ^{[1
]}

机构：

[1] Univ Manitoba, Dept Biol Sci, Winnipeg, MB, Canada

[2] US Fish & Wildlife Serv, Columbia River Fisheries Program Off, Vancouver, WA USA

来源：

GENETICS AND MOLECULAR RESEARCH | 2011年 / 10卷 / 04期

关键词：

Entosphenus; Lampetra richardsoni; Microsatellite; Pacific lamprey; Petromyzontidae; Western brook lamprey;

D O I：

10.4238/2011.December.22.2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Pacific lamprey (Entosphenus tridentatus) is an anadromous fish that is of conservation concern in North America and Asia. Data on Pacific lamprey population structure are scarce and conflicting, impeding conservation efforts. We optimized 12 polymorphic microsatellite loci for the Pacific lamprey. Three to 13 alleles per locus were observed in a sample of 51 fish collected from the West Fork Illinois River, Oregon. Observed heterozygosity ranged from 0.235 to 0.902 and expected heterozygosity ranged from 0.214 to 0.750. Cross-species amplification produced 8 to 12 polymorphic loci in four other Entosphenus species and in the western brook lamprey (Lampetra richardsoni). Two loci appear to be diagnostic for distinguishing Entosphenus from Lampetra. These markers will be valuable for evaluating population structure and making conservation decisions for E. tridentatus and other lamprey species.

引用

页码：3246 / 3250

页数：5

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