Inhibition of Proliferation, Migration, and Invasion by Knockdown of Pyruvate Kinase-M2 (PKM2) in Ovarian Cancer SKOV3 and OVCAR3 Cells

被引：31
作者：
Miao, Yi [1 ]
Lu, Meng [1 ]
Yan, Qin [1 ]
Li, Shuangdi [1 ]
Feng, Youji [1 ]
机构：
[1] Nanjing Med Univ, Shanghai Gen Hosp, Dept Obstet & Gynecol, 100 Haining Rd, Shanghai 200080, Peoples R China
来源：
ONCOLOGY RESEARCH | 2016年 / 24卷 / 06期
关键词：
Pyruvate kinase isozyme type M2 (PKM2); Ovarian carcinoma (OC); Cell proliferation; Metastasis; Signaling pathway; TUMOR-CELLS; COLORECTAL-CANCER; M2; METABOLISM; PROTEIN; GROWTH; GLYCOLYSIS; CARCINOMAS; EXPRESSION; REGULATOR;
D O I：
10.3727/096504016X14685034103671
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Pyruvate kinase (PK) is a key enzyme in the process of glycolysis, catalyzing phosphoenolpyruvate (PEP) into pyruvate. Currently, PK isozyme type M2 (PKM2), one subtype of PK, has been proposed as a new tumor marker with high expression in various tumor tissues. Here we aimed to explore the effects of siRNA-PKM2 on ovarian carcinoma (OC) cell lines SKOV3 and OVCAR3, in which PKM2 was notably expressed. PKM2 gene interference lentivirus vectors were built by miRNA transfection assay. siRNA-PKM2-transfected SKOV3 and OVCAR3 cells were evaluated for cell proliferation, cell cycle distribution, cell apoptosis, cell migration, and invasion in this study. In addition, the expression levels of several tumor-related genes were measured using real-time PCR and Western blot. Results showed that siRNA-PKM2 markedly inhibited cell proliferation, induced apoptosis, and caused cell cycle arrest at the G(0)/G(1) phase. Cell migration and invasion were significantly suppressed by siRNA-PKM2. Furthermore, the tumor-related genes caspase 7, Bad, and E-cadherin were upregulated, while MMP2, HIF1 alpha, VEGF, and MMP9 were depressed by siRNA-PKM2. The function of siRNA-PKM2 on the biological behavior of OC cells indicated that PKM2 may also be a target for treatment of OC.
引用
收藏
页码：463 / 475
页数：13
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