Determination of type and concentration of DNA nitrogenous bases by Raman spectroscopy

被引：3
|
作者：
Laptinskiy, Kirill A. [1 ]
Burikov, Sergey A. [1 ]
Dolenko, Tatiana A. [1 ]
机构：
[1] Moscow MV Lomonosov State Univ, Dept Phys, Moscow 119991, Russia
来源：
SARATOV FALL MEETING 2014: OPTICAL TECHNOLOGIES IN BIOPHYSICS AND MEDICINE XVI; LASER PHYSICS AND PHOTONICS XVI; AND COMPUTATIONAL BIOPHYSICS | 2015年 / 9448卷
关键词：
DNA chain; molecular computations; nitrogenous bases; Raman spectroscopy; NUCLEIC-ACIDS; COMPUTATION; LIGATION; FIDELITY;
D O I：
10.1117/12.2180055
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
In this paper results of application of laser Raman spectroscopy for increasing reliability of molecular DNA computations are presented. It is shown that elaborated method provides the accuracy of determination of concentration of individual nitrogenous bases 0.03 g/l and the accuracy of determination of total DNA concentration in the solutions 0.04 g/l by Raman spectra.
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页数：8
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