Origin of embryo-like structures in soybean anther culture investigated using SSR marker

被引：18

作者：

Rodrigues, LR ^{[1
]}

Terra, TD ^{[1
]}

Bered, F ^{[1
]}

Bodanese-Zanettini, MH ^{[1
]}

机构：

[1] Univ Fed Rio Grande Sul, Inst Biociencias, Dept Genet, BR-91501970 Porto Alegre, RS, Brazil

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 2004年 / 77卷 / 03期

关键词：

androgenesis; anther culture; Glycine max; microsatellite; somatic embryogenesis;

D O I：

10.1023/B:TICU.0000018397.26315.17

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The Satt418 microsatellite locus was examined in order to investigate the origin of embryo-like structures (ELS) obtained from soybean anther culture. Four heterozygous plants were used as anther donors. A total of 7000 anthers were placed on the induction medium under culture conditions known to trigger androgenic response. After 60 days of culture, upper portion of 216 ELS were carefully removed and transferred to a proliferation medium, in order to obtain sufficient tissue for DNA extraction. Callogenic masses originated from 114 ELS were screened for the Satt418 microsatellite locus. Heterozygous and homozygous ELS were identified, suggesting occurrence of somatic embryogenesis and androgenesis in the same system. This unexpected morphogenic response seems to be genotype-dependent.

引用

页码：287 / 289

页数：3

共 7 条

[1]

Doyle J. L., 1987, Phytochemical Bulletin, V19, P11, DOI 10.2307/4119796

[2]

Hu CY., 1996, Vitro haploid production in higher plants, current plant science and biotechnology in agriculture, P377

[3] Cytological analysis of early microspore divisions and embryo formation in cultured soybean anthers [J].

KaltchukSantos, E ;

Mariath, JE ;

Mundstock, E ;

Hu, CY ;

BodaneseZanettini, MH .

PLANT CELL TISSUE AND ORGAN CULTURE, 1997, 49 (02) :107-115

[4] Development of simple sequence repeat DNA markers and their integration into a barley linkage map [J].

Liu, ZW ;

Biyashev, RM ;

Maroof, MAS .

THEORETICAL AND APPLIED GENETICS, 1996, 93 (5-6) :869-876

[5]

Ye XinGuo, 1994, Soybean Science, V13, P193

[6]

Yin GC., 1982, Soybean Sci, V1, P69

[7] Germination of embryo in soybean anther culture [J].

Zhao, GL ;

Liu, YZ ;

Yin, AP ;

Li, JB .

CHINESE SCIENCE BULLETIN, 1998, 43 (23) :1991-1995

← 1 →