Lipidomic and transcriptomic analysis reveals reallocation of carbon flux from cuticular wax into plastid membrane lipids in a glossy "Newhall" navel orange mutant

被引:25
作者
Wan, Haoliang [1 ]
Liu, Hongbo [2 ]
Zhang, Jingyu [1 ]
Lyu, Yi [3 ]
Li, Zhuoran [1 ]
He, Yizhong [1 ]
Zhang, Xiaoliang [1 ]
Deng, Xiuxin [1 ]
Brotman, Yariv [4 ]
Fernie, Alisdair R. [5 ]
Cheng, Yunjiang [1 ]
Wen, Weiwei [1 ]
机构
[1] Huazhong Agr Univ, Coll Hort & Forestry Sci, Key Lab Hort Plant Biol MOE, Wuhan 430070, Peoples R China
[2] Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China
[3] Northwestern Polytech Univ, Sch Life Sci, Key Lab Space Biosci & Biotechnol, Youyi Xilu 127, Xian 710072, Shaanxi, Peoples R China
[4] Ben Gurion Univ Negev, Dept Life Sci, Beer Sheva, Israel
[5] Max Planck Inst Mol Plant Physiol, Muehlenberg 1, D-14476 Potsdam, Germany
基金
中国国家自然科学基金;
关键词
JASMONIC ACID; CITRUS-SINENSIS; FATTY-ACID; CUTICLE FORMATION; BIOSYNTHESIS; FRUIT; GENE; OVEREXPRESSION; ULTRASTRUCTURE; RESISTANCE;
D O I
10.1038/s41438-020-0262-z
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Both cuticle and membrane lipids play essential roles in quality maintenance and disease resistance in fresh fruits. Many reports have indicated the modification of alternative branch pathways in epicuticular wax mutants; however, the specific alterations concerning lipids have not been clarified thus far. Here, we conducted a comprehensive, time-resolved lipidomic, and transcriptomic analysis on the "Newhall" navel orange (WT) and its glossy mutant (MT) "Gannan No. 1". The results revealed severely suppressed wax formation accompanied by significantly elevated production of 36-carbon plastid lipids with increasing fruit maturation in MT. Transcriptomics analysis further identified a series of key functional enzymes and transcription factors putatively involved in the biosynthesis pathways of wax and membrane lipids. Moreover, the high accumulation of jasmonic acid (JA) in MT was possibly due to the need to maintain plastid lipid homeostasis, as the expression levels of two significantly upregulated lipases (CsDAD1 and CsDALL2) were positively correlated with plastid lipids and characterized to hydrolyze plastid lipids to increase the JA content. Our results will provide new insights into the molecular mechanisms underlying the natural variation of plant lipids to lay a foundation for the quality improvement of citrus fruit.
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页数:18
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