A highly conserved cryptic epitope in the receptor binding domains of SARS-CoV-2 and SARS-CoV

被引：1092

作者：

Yuan, Meng ^{[1
]}

Wu, Nicholas C. ^{[1
]}

Zhu, Xueyong ^{[1
]}

Lee, Chang-Chun D. ^{[1
]}

So, Ray T. Y. ^{[2
]}

Lv, Huibin ^{[2
]}

Mok, Chris K. P. ^{[2
]}

Wilson, Ian A. ^{[1
,3
]}

机构：

[1] Scripps Res Inst, Dept Integrat Struct & Computat Biol, La Jolla, CA 92037 USA

[2] Univ Hong Kong, La Ka Shiny Fac Med, Sch Publ Hlth, HKU Pasteur Res Pole, Hong Kong, Peoples R China

[3] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA

来源：

SCIENCE | 2020年 / 368卷 / 6491期

基金：

中国国家自然科学基金; 美国国家卫生研究院;

关键词：

MONOCLONAL-ANTIBODY; SPIKE PROTEIN; CORONAVIRUS; NEUTRALIZATION;

D O I：

10.1126/science.abb7269

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The outbreak of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) has now become a pandemic, but there is currently very little understanding of the antigenicity of the virus. We therefore determined the crystal structure of CR3022, a neutralizing antibody previously isolated from a convalescent SARS patient, in complex with the receptor binding domain (RBD) of the SARS-CoV-2 spike (S) protein at 3.1-angstrom resolution. CR3022 targets a highly conserved epitope, distal from the receptor binding site, that enables cross-reactive binding between SARS-CoV-2 and SARS-CoV. Structural modeling further demonstrates that the binding epitope can only be accessed by CR3022 when at least two RBDs on the trimeric S protein are in the "up" conformation and slightly rotated. These results provide molecular insights into antibody recognition of SARS-CoV-2.

引用

页码：630 / +

页数：27

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