Recombinant leptin in the hypothalamic response to late fasting

被引：11

作者：

Bertile, F ^{[1
]}

Oudart, H ^{[1
]}

Le Maho, Y ^{[1
]}

Raclot, T ^{[1
]}

机构：

[1] Univ Strasbourg 1, CNRS, UPR 9010, Ctr Ecol & Physiol Energet, F-67087 Strasbourg 2, France

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2003年 / 310卷 / 03期

关键词：

hypothalamic neuropeptides; gene expression; leptin perfusion; long-term food deprivation; refeeding signal;

D O I：

10.1016/j.bbrc.2003.09.117

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of the current study was to gain further insight into the implication of leptin in the regulation of hypothalamic gene expression during long-term food deprivation with emphasis on phase 3 of fasting (P3, late protein breakdown). Among plasma parameters, glucose, non-esterified fatty acids, and insulin levels tended to be decreased by leptin infusion, whilst corticosterone levels remained unchanged. From Northern blot analysis, NPY, AGRP, and MCH mRNA gene expressions were differentially regulated during prolonged fasting in leptin-perfused rats. In comparison with fed animals, NPY, AGRP, and MCH mRNA levels in P3 rats treated with leptin either remained stable or increased slightly. Regarding anorexigenic peptides (CART and POMC) and prepro-OX, fasting with leptin induced only slight changes in gene expression. Similar data have been obtained in leptin-treated fasted rats at various doses within the physiological range. We conclude that leptin and particularly low levels of plasma leptin can reasonably be considered as a constituent of a signal triggering the fasting-induced enhanced drive for refeeding in P3. (C) 2003 Elsevier Inc. All rights reserved.

引用

页码：949 / 955

页数：7

共 33 条

[1] Distinct physiologic and neuronal responses to decreased leptin and mild hyperleptinemia [J].

Ahima, RS ;

Kelly, J ;

Elmquist, JK ;

Flier, JS .

ENDOCRINOLOGY, 1999, 140 (11) :4923-4931

[2] Leptin [J].

Ahima, RS ;

Flier, JS .

ANNUAL REVIEW OF PHYSIOLOGY, 2000, 62 :413-437

[3] Role of leptin in the neuroendocrine response to fasting [J].

Ahima, RS ;

Prabakaran, D ;

Mantzoros, C ;

Qu, DQ ;

Lowell, B ;

MaratosFlier, E ;

Flier, JS .

NATURE, 1996, 382 (6588) :250-252

[4] Insulin and leptin combine additively to reduce food intake and body weight in rats [J].

Air, EL ;

Benoit, SC ;

Clegg, DJ ;

Seeley, RJ ;

Woods, SC .

ENDOCRINOLOGY, 2002, 143 (06) :2449-2452

[5] Hypothalamic gene expression in long-term fasted rats: relationship with body fat [J].

Bertile, F ;

Oudart, H ;

Criscuolo, F ;

Le Maho, Y ;

Raclot, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2003, 303 (04) :1106-1113

[6] The neuropeptide Y agouti gene-related protein (AGRP) brain circuitry in normal, anorectic, and monosodium glutamate-treated mice [J].

Broberger, C ;

Johansen, J ;

Johansson, C ;

Schalling, M ;

Hökfelt, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (25) :15043-15048

[7] INVOLVEMENT OF CORTICOSTERONE IN THE FASTING-INDUCED RISE IN PROTEIN-UTILIZATION AND LOCOMOTOR-ACTIVITY [J].

CHALLET, E ;

LEMAHO, Y ;

ROBIN, JP ;

MALAN, A ;

CHEREL, Y .

PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR, 1995, 50 (03) :405-412

[8] INVIVO GLUCOSE-UTILIZATION IN RAT-TISSUES DURING THE 3 PHASES OF STARVATION [J].

CHEREL, Y ;

BURNOL, AF ;

LETURQUE, A ;

LEMAHO, Y .

METABOLISM-CLINICAL AND EXPERIMENTAL, 1988, 37 (11) :1033-1039

[9] REFEEDING AFTER THE LATE INCREASE IN NITROGEN-EXCRETION DURING PROLONGED FASTING IN THE RAT [J].

CHEREL, Y ;

LEMAHO, Y .

PHYSIOLOGY & BEHAVIOR, 1991, 50 (02) :345-349

[10] SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].

CHOMCZYNSKI, P ;

SACCHI, N .

ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159

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