Profiling eicosanoids and phospholipids using LC-MS/MS: Principles and recent applications

被引:23
作者
Ecker, Josef [1 ]
机构
[1] ABF Analytisch Biol Forschungslab GmbH, D-80336 Munich, Germany
关键词
Eicosanoid; Phospholipid; Lipid mediator; Lipidomics; LC-MS; MS; TANDEM MASS-SPECTROMETRY; QUANTITATIVE-ANALYSIS; ELECTROSPRAY-IONIZATION; URINARY PHOSPHOLIPIDS; SHOTGUN LIPIDOMICS; BREAST-CANCER; ARACHIDONIC-ACID; MEMBRANE-LIPIDS; SOFTWARE TOOLS; FATTY-ACIDS;
D O I
10.1002/jssc.201200056
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Eicosanoids are potent lipid mediators involved in numerous physiological and pathophysiological processes. Precursors are polyunsaturated fatty acids liberated from membrane phospholipids. Thus, profiling and quantification of these molecules has gained a lot of attention during last years. Eicosanoids and phospholipids are commonly profiled by LC-MS/MSbecause this technique allows accurate quantification within acceptable run-times. This article therefore focuses on liquid chromatography and the ESI-MS/MS analysis of proinflammatory lipid mediators, particularly arachidonic acid (C20:4) derived eicosanoids and their precursors phospholipids. Recent analytical developments for quantification of these compounds are highlighted and analytical challenges are discussed. Furthermore, applications such as the use of these molecules as biomarkers are presented.
引用
收藏
页码:1227 / 1235
页数:9
相关论文
共 79 条
  • [1] Ion suppression in mass spectrometry
    Annesley, TM
    [J]. CLINICAL CHEMISTRY, 2003, 49 (07) : 1041 - 1044
  • [2] Stereochemical assignment, antiinflammatory properties, and receptor for the omega-3 lipid mediator resolvin E1
    Arita, M
    Bianchini, F
    Aliberti, J
    Sher, A
    Chiang, N
    Hong, S
    Yang, R
    Petasis, NA
    Serhan, CN
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 2005, 201 (05) : 713 - 722
  • [3] Metabolic profiling of glycerophospholipid synthesis in fibroblasts loaded with free cholesterol and modified low density lipoproteins
    Binder, Marion
    Liebisch, Gerhard
    Langmann, Thomas
    Schmitz, Gerd
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (31) : 21869 - 21877
  • [4] BLIGH EG, 1959, CAN J BIOCHEM PHYS, V37, P911
  • [5] LC/MS/MS method for analysis of E2 series prostaglandins and isoprostanes
    Brose, Stephen A.
    Thuen, Brock T.
    Golovko, Mikhail Y.
    [J]. JOURNAL OF LIPID RESEARCH, 2011, 52 (04) : 850 - 859
  • [6] Liquid chromatographic-mass spectrometric analysis of phospholipids. Chromatography, ionization and quantification
    Brouwers, Jos F.
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 2011, 1811 (11): : 763 - 775
  • [7] Quantitative analysis of biological membrane lipids at the low picomole level by nano-electrospray ionization tandem mass spectrometry
    Brugger, B
    Erben, G
    Sandhoff, R
    Wieland, FT
    Lehmann, WD
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (06) : 2339 - 2344
  • [8] An integrated omics analysis of eicosanoid biology
    Buczynski, Matthew W.
    Dumlao, Darren S.
    Dennis, Edward A.
    [J]. JOURNAL OF LIPID RESEARCH, 2009, 50 (06) : 1015 - 1038
  • [9] BJN to publish more issues and more papers in 2009
    Calder, Philip C.
    [J]. BRITISH JOURNAL OF NUTRITION, 2009, 101 (01) : 1 - 1
  • [10] Polyunsaturated fatty acids and inflammatory processes: New twists in an old tale
    Calder, Philip C.
    [J]. BIOCHIMIE, 2009, 91 (06) : 791 - 795