Synthesis of alkaline protease by catabolite repression-resistant Thermoactinomyces sp. E79 mutant

被引：7

作者：

Sunitha, K

Park, YS

Oh, TK

Lee, JK

机构：

[1] Korea Res Inst Biosci & Biotechnol, Microbial Enzyme Res Unit, Taejon 305600, South Korea

[2] Kyungwon Univ, Dept Food & Bioengn, Sungnam, South Korea

来源：

BIOTECHNOLOGY LETTERS | 1999年 / 21卷 / 02期

关键词：

catabolite repression; mutation; glucose transport; protease; Thermoactinomyces;

D O I：

10.1023/A:1005479508156

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The production of alkaline protease from Thermoactinomyces sp. E79 was repressed by 0.2% (w/v) glucose in the medium. Catabolite repression-resistant mutant M1 was obtained by combined treatment of UV light and N-methyl-N'-nitro-N-nitrosoguanidine. The glucose uptake studies accomplished by [C-14]glucose showed that the mutant has lost its ability for glucose uptake. The protease production by mutant M1 in the enzyme production medium was 62 U/mg, which was twice that of the wild-type strain.

引用

页码：155 / 158

页数：4

共 10 条

[1]

ALEA F, 1991, APPL MICROBIOL BIOT, V35, P643, DOI 10.1007/BF00169630

[2]

BIERBAUM G, 1994, APPL MICROBIOL BIOT, V40, P611, DOI 10.1007/BF00173316

[3] THERMOPHILIC PROTEASES - PROPERTIES AND POTENTIAL APPLICATIONS [J].