Inhibited experimental corneal neovascularization by neutralizing anti-SDF-1α antibody

被引：9
作者：
Liu, Gao-Qin [1 ,2 ]
Lu, Pei-Rong [1 ,2 ]
Li, Long-Biao [1 ]
Zhang, Xue-Guang [2 ]
机构：
[1] Soochow Univ, Dept Ophthalmol, Affiliated Hosp 1, Suzhou 215006, Jiangsu, Peoples R China
[2] Soochow Univ, Jiangsu Inst Clin Immunol, Affiliated Hosp 1, Suzhou 215006, Jiangsu, Peoples R China
来源：
INTERNATIONAL JOURNAL OF OPHTHALMOLOGY | 2012年 / 5卷 / 01期
关键词：
corneal neovascularization; alkali injury; chemokine; ENDOTHELIAL GROWTH-FACTOR; CELL-DERIVED FACTOR; CXCR4; CHEMOATTRACTANT; ANGIOGENESIS; FACTOR-1;
D O I：
10.3980/j.issn.2222-3959.2012.01.02
中图分类号：
R77 [眼科学];
学科分类号：
100212 ;
摘要：
AIM: To explore the effect of SDF-1 alpha on the development of experimental corneal neovascularization (CRNV). METHODS: CRNV was induced by alkali injury in mice. The expression of SDF-1 alpha and CXCR4 in burned corneas was examined by Flow Cytometry. Neutralizing anti-mouse SDF-1 alpha antibody was locally administrated after alkali injury and the formation of CRNV 2 weeks after injury was assessed by Immunohistochemistry. The expression of VEGF and C-Kit in burned corneas was detected by RT-PCR. RESULTS: The number of CRNV peaks at 2 weeks after alkali injury. Compared to control group, SDF-1 alpha neutralizing antibody treatment significantly decreased the number of CRNV. RT-PCR confirmed that SDF-1 alpha neutralizing antibody treatment resulted in decreased intracorneal VEGF and C-Kit expression. CONCLUSION: SDF-1 alpha neutralizing antibody treated mice exhibited impaired experimental CRNV through down regulated VEGF and C-Kit expression.
引用
收藏
页码：7 / 12
页数：6
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